New Diagnostic Methods Department, Russian Cardiology Research Complex, 3rd Cherepkovskaya Street, 15a, Moscow 121552, Russia.
Microvasc Res. 2012 Jul;84(1):86-93. doi: 10.1016/j.mvr.2012.03.011. Epub 2012 Apr 4.
Correlation between metabolic and microhemodynamic processes in skin was assessed through acute pharmacological test with metabolically active Actovegin in 28 healthy volunteers. Laser Doppler flowmetry in combination with wavelet analysis of blood flow oscillations was used to identify functional state of arteriolar-venular areas of microvascular bed in the right forearm skin; capillary blood flow parameters were assessed through computer capillaroscopy in the nail bed of the right hand on the 4th finger. The metabolic effect (improved oxygen uptake and glucose disposal by tissues) was accompanied by significant increase in endothelial rhythm amplitude by 98% (p<0.00006), neurogenic rhythm amplitude by 50% (p<0.003) and myogenic rhythm amplitude by 54% (p<0.03), with capillary blood flow rate increasing by 90μm/s (p<0.04), pericapillary zone reducing by 15μm (p<0.0001) and diastolic blood pressure dropping by 4mm Hg (p<0.02). These results show close correlation between metabolic and microhemodynamic processes, which suggests that the amplitude activity within the range of endothelial rhythm (0.0095-0.021Hz) during laser Doppler flowmetry reflects not only solely vasomotor function but also metabolic function of microvascular endothelium.
通过对 28 名健康志愿者进行具有代谢活性的 Actovegin 的急性药理学测试，评估了皮肤代谢和微循环过程之间的相关性。激光多普勒血流仪结合血流振荡的小波分析，用于识别右前臂皮肤微血管床的动静脉区域的功能状态；通过右手第四指指甲床的计算机毛细血管镜评估毛细血管血流参数。代谢效应（改善组织的氧气摄取和葡萄糖处理）伴随着内皮节律幅度显著增加 98%（p<0.00006），神经节律幅度增加 50%（p<0.003），肌源性节律幅度增加 54%（p<0.03），毛细血管血流速度增加 90μm/s（p<0.04），毛细血管周围区域减少 15μm（p<0.0001），舒张压下降 4mmHg（p<0.02）。这些结果表明代谢和微循环过程之间存在密切相关性，这表明激光多普勒血流仪内皮节律范围内的幅度活动不仅反映了单纯的血管舒缩功能，还反映了微血管内皮的代谢功能。
