Mangin M, Ikeda K, Broadus A E
Department of Internal Medicine, Yale University, New Haven, CT 06510.
Gene. 1990 Nov 15;95(2):195-202. doi: 10.1016/0378-1119(90)90362-u.
The parathyroid hormone-related peptide (PTHRP) was initially isolated from tumors associated with the syndrome of humoral hypercalcemia of malignancy. The human PTHRP gene is a complex transcriptional unit which uses multiple promoters and contains alternatively spliced 3' exons that result in mRNAs encoding three different deduced products. We report here the structure of the mouse PTHRP gene. The mouse gene has a considerably simpler organization than its human counterpart. This organization includes a single 3' exon and an apparent single 3' splicing pathway, leading to an mRNA encoding a 139-amino acid mature PTHRP. In addition, the mouse gene appears to be predominantly under the control of a short proximal promoter element. By RNase protection analysis, we identified PTHRP mRNA in specimens prepared from a variety of normal rodent tissues, including a number of tissues not previously recognized as sites of PTHRP gene expression.
甲状旁腺激素相关肽（PTHRP）最初是从与恶性肿瘤体液性高钙血症综合征相关的肿瘤中分离出来的。人类PTHRP基因是一个复杂的转录单位，它使用多个启动子，并包含选择性剪接的3'外显子，这些外显子产生编码三种不同推导产物的mRNA。我们在此报告小鼠PTHRP基因的结构。小鼠基因的组织比其人类对应物要简单得多。这种组织包括一个单一的3'外显子和一条明显单一的3'剪接途径，导致编码139个氨基酸的成熟PTHRP的mRNA。此外，小鼠基因似乎主要受一个短的近端启动子元件的控制。通过核糖核酸酶保护分析，我们在从各种正常啮齿动物组织制备的标本中鉴定出了PTHRP mRNA，包括一些以前未被认为是PTHRP基因表达位点的组织。
