反向注射毛细管电泳紫外检测法测定人全血中的血清素。

Reverse injection capillary electrophoresis UV detection for serotonin quantification in human whole blood.

机构信息

Dept. Biomedical Sciences, University of Sassari, Italy.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2012 May 1;895-896:182-5. doi: 10.1016/j.jchromb.2012.03.024. Epub 2012 Mar 26.

DOI:10.1016/j.jchromb.2012.03.024

PMID:22497835

Abstract

We describe the first capillary electrophoresis UV detection method to measure serotonin in human whole blood (WB). Procedural parameters such as concentration and pH of run buffer and injection mode were investigated. The reverse injection allows to decrease the analysis time by injecting samples at the outlet end of the silica capillary close to the detection window, so reducing the migration distance. Thus, when a capillary with an effective length of 10 cm and a 400 mmol/L Tris phosphate as background electrolyte at pH 3.25 was used, the migration time of the serotonin peak was 2.6 min. These conditions gave a good reproducibility of migration times (CV, 0.77%) and peak areas (CV, 2.44%). Intra- and inter-assay CV were 3.85% and 7.32%, respectively, and the analytical recovery was between 96.8% and 99.4%.

摘要

我们描述了一种用于测量人全血中血清素的毛细管电泳紫外检测法。考察了运行缓冲液的浓度和 pH 值以及进样模式等程序参数。反向进样允许通过在靠近检测窗口的二氧化硅毛细管出口端进样来减少分析时间，从而缩短迁移距离。因此，当使用有效长度为 10 cm 的毛细管和 400 mmol/L 的三磷酸氢盐作为 pH 值为 3.25 的背景电解质时，血清素峰的迁移时间为 2.6 分钟。在这些条件下，迁移时间（CV，0.77%）和峰面积（CV，2.44%）具有良好的重现性。内标和间标 CV 分别为 3.85%和 7.32%，分析回收率在 96.8%至 99.4%之间。