Instituto de la Grasa (CSIC), Avda Padre García Tejero 4, 41012-Sevilla, Spain.
Food Chem Toxicol. 2012 Jun;50(6):1951-4. doi: 10.1016/j.fct.2012.03.071. Epub 2012 Apr 1.
The erythrocyte agglutinating activity of polyphenol extracts from six grain legumes was investigated. Polyphenols are amphipathic molecules that can bind to proteins and lipids through hydrophobic and polar interactions, leading to agglutination of liposomes and bacteria. The extracts from four of the six legumes that were studied caused erythrocyte agglutination at concentrations in the μM range. Soybean extracts had the highest activity, followed by the extracts from lentils, broad bean, and chickpea. As a good representative of these legumes, binding of the polyphenols extracted from lentils to erythrocytes was investigated in more detail, showing that agglutination was mediated by binding of 84% of the polyphenols present in the incubation, which corresponds to 2.42 μg bound polyphenols/mg erythrocytes, and a maximum polyphenol binding of 96% according to Lineweaver-Burk plots. The relatively high concentrations that are required for agglutination justify that polyphenols more probably do not agglutinate erythrocytes in vivo, but the possibility still exists that in vivo binding without agglutination could occur, which could have some effects on the metabolism and health-promoting properties of polyphenols.
研究了六种豆科植物多酚提取物的红细胞凝集活性。多酚是两亲性分子，可通过疏水和极性相互作用与蛋白质和脂质结合，导致脂质体和细菌的凝集。在所研究的六种豆科植物的四种提取物中，在μM 浓度范围内引起红细胞凝集。大豆提取物的活性最高，其次是小扁豆、蚕豆和鹰嘴豆的提取物。作为这些豆科植物的良好代表，更详细地研究了从小扁豆中提取的多酚与红细胞的结合，表明凝集是通过结合存在于孵育液中的 84%的多酚介导的，这相当于 2.42μg 结合的多酚/mg 红细胞，根据 Lineweaver-Burk 图谱，多酚的最大结合率为 96%。凝集所需的相对较高浓度表明多酚在体内不太可能凝集红细胞，但仍存在体内结合而不凝集的可能性，这可能对多酚的代谢和促进健康的特性产生一些影响。
