Quiroz-Figueroa Francisco R, Monribot-Villanueva Juan L, Bojórquez-Velázquez Esaú, Gómez-Peraza Rosa L, Elizalde-Contreras José M, Bautista-Valle Mirna V, Guerrero-Analco José A, Valdez-Morales Maribel, Singh Rupesh Kumar, Ruiz-May Eliel
Laboratorio de Fitomejoramiento Molecular, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional-Unidad Sinaloa, Instituto Politécnico Nacional, Boulevard Juan de Dios Bátiz Paredes # 250, Col. San Joachin, Guasave 81101, Sinaloa, Mexico.
Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Cluster BioMimic®, Carretera Antigua a Coatepec 351, Congregación el Haya, Xalapa 91073, Veracruz, Mexico.
Antioxidants (Basel). 2022 Sep 20;11(10):1850. doi: 10.3390/antiox11101850.
Legumes are an essential source of nutrients that complement energy and protein requirements in the human diet. They also contribute to the intake of bioactive compounds such as polyphenols, whose content can vary depending on cultivars and genotypes. We conducted a comparative proteomics and metabolomics study to determine if there were significant variations in relevant nutraceutical compounds in the five genotypes of Kabuli-type chickpea grains. We performed an isobaric tandem mass tag (TMT) couple to synchronous precursor selection (SPS)-MS3 method along with a targeted and untargeted metabolomics approach based on accurate mass spectrometry. We observed an association between the overproduction of proteins involved in starch, lipid, and amino acid metabolism with gibberellin accumulation in large grains. In contrast, we visualized the over-accumulation of proteins associated with water deprivation in small grains. It was possible to visualize in small grains the over-accumulation of some phenolics such as vanillin, salicylic acid, protocatechuic acid, 4-coumaric acid, 4-hydroxybenzoic acid, vanillic acid, ferulic acid, and kaempferol 3--glucoside as well as the amino acid l-phenylalanine. The activated phenolic pathway was associated with the higher antioxidant capacity of small grains. Small grains consumption could be advantageous due to their nutraceutical properties.
豆类是人类饮食中补充能量和蛋白质需求的重要营养来源。它们还能增加生物活性化合物的摄入量，如多酚，其含量会因品种和基因型而异。我们进行了一项比较蛋白质组学和代谢组学研究，以确定卡布利型鹰嘴豆五个基因型的相关营养化合物是否存在显著差异。我们采用了等压串联质量标签（TMT）与同步前体选择（SPS）-MS3相结合的方法，以及基于精确质谱的靶向和非靶向代谢组学方法。我们观察到，大粒种子中参与淀粉、脂质和氨基酸代谢的蛋白质过量产生与赤霉素积累之间存在关联。相反，我们发现小粒种子中与水分缺乏相关的蛋白质过度积累。在小粒种子中可以看到一些酚类物质的过度积累，如香草醛、水杨酸、原儿茶酸、对香豆酸、4-羟基苯甲酸、香草酸、阿魏酸和山奈酚3-O-葡萄糖苷，以及氨基酸L-苯丙氨酸。激活的酚类途径与小粒种子较高的抗氧化能力有关。由于其营养特性，食用小粒种子可能具有优势。
