采用单克隆抗Le(a)和抗Le(b)抗体，通过酶联免疫吸附测定（ELISA）对人唾液斑进行Lewis血型分型。
Lewis typing of human saliva stains by enzyme-linked immunosorbent assay (ELISA) using monoclonal anti-Le(a) and anti-Le(b) antibodies.
作者信息
Takatori T, Tsutsubuchi Y, Terazawa K, Nagao M, Akabame H, Mikami H
机构信息
Department of Legal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
出版信息
Forensic Sci Int. 1990 Oct;47(3):261-8. doi: 10.1016/0379-0738(90)90295-a.
The Lewis blood grouping of human saliva stains could be detected by an enzyme-linked immunosorbent assay (ELISA) using anti-Le(a) and anti-Le(b) monoclonal antibodies with an avidin-biotin complex (ABC). The saliva stains (1.0 by 1.0 cm in size) were used as samples and not only could the Lewis substances of 57 individual stains be correctly typed by this method, but also it was clarified that there are several different secretion patterns of amounts of Le(a) and Le(b) substances in 3 individual Lewis types.
人唾液斑的Lewis血型可通过酶联免疫吸附测定（ELISA）检测，该方法使用抗Le(a)和抗Le(b)单克隆抗体与抗生物素蛋白-生物素复合物（ABC）。将大小为1.0×1.0厘米的唾液斑用作样本，不仅可以通过该方法正确鉴定57个个体斑的Lewis物质，而且还明确了3种个体Lewis血型中Le(a)和Le(b)物质的量存在几种不同的分泌模式。
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