Jones D G, Kay A B
Int Arch Allergy Appl Immunol. 1977;55(1-6):277-82. doi: 10.1159/000231937.
Histamine and one of its major metabolites, imidazoleacetic acid, were selectively chemotactic for guinea pig eosinophils, whereas L-histidine, 1,4-methylimidazoleacetic acid, 1,4-methylhistamine and N-acetylhistamine were inactive. The response to histamine was unaffected by concentrations of eosinophils of between 30 and greater than 90% but it was abrogated by preincubation of the cells with histamine prior to assay (self-deactivation). Eosinophilotaxis was also inhibited by H1-(mepyramine-) and H2-)burimamide)-receptor antagonists at high doses (10(-3)m), although at lower concentrations (10(-5) M) inhibition was principally associated with burimamide. The human tetrapeptide, alanine-glycine-serine-glutamic acid, and the analogue, valine-glycine-aspartic acid-glutamic acid, were inactive whereas alanine-glycine-serine-glutamic acid was chemotactic for the guinea pig eosinophil. These results support the concept that the tissue accumulation of eosinophils following anaphylaxis depends on a complex interaction of factors, which in part may be mediated by H2 receptors on the target cells. There may be species differences in the composition of ECF-A.
组胺及其主要代谢产物之一咪唑乙酸对豚鼠嗜酸性粒细胞具有选择性趋化作用，而L-组氨酸、1,4-甲基咪唑乙酸、1,4-甲基组胺和N-乙酰组胺则无活性。对组胺的反应不受嗜酸性粒细胞浓度在30%至大于90%之间的影响，但在测定前用组胺对细胞进行预孵育（自身失活）可消除该反应。高剂量（10⁻³m）的H1-（美吡拉敏）和H2-（布立马胺）受体拮抗剂也可抑制嗜酸性粒细胞趋化作用，尽管在较低浓度（10⁻⁵M）时，抑制作用主要与布立马胺有关。人四肽丙氨酸-甘氨酸-丝氨酸-谷氨酸及其类似物缬氨酸-甘氨酸-天冬氨酸-谷氨酸无活性，而丙氨酸-甘氨酸-丝氨酸-谷氨酸对豚鼠嗜酸性粒细胞具有趋化作用。这些结果支持这样一种概念，即过敏反应后嗜酸性粒细胞在组织中的积聚取决于多种因素的复杂相互作用，其中部分因素可能由靶细胞上的H2受体介导。嗜酸性粒细胞趋化因子A（ECF-A）的组成可能存在种属差异。
