Beijing National Laboratory for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
J Colloid Interface Sci. 2012 Jul 1;377(1):34-9. doi: 10.1016/j.jcis.2012.03.055. Epub 2012 Mar 28.
Nano/microstructures play an important role in nanoparticle applications. This paper describes an innovative strategy to fabricate a variety of gold nanoparticle aggregates (AuNPs) on large-scale arrays of up to ∼1 cm(2) made from polystyrene (PS). A dendritic surfactant, C18N3, has multi-amine head groups that can control the thickness of a double layer adsorbed on the PS sphere surface in a pH-dependent manner. Controlling the pH and immersion time in the C18N3 solution allows the morphology of AuNPs deposited on the PS spheres (PS@AuNP) to be regulated. The influence of nano/microstructures on the activity enhancement of glucose oxidase (GOD) was investigated. The results indicated that well-ordered PS@AuNP arrays performed much better in the specific activity enhancement of GOD compared with free GOD and GOD immobilized on PS arrays. Furthermore, it was observed that the immobilized GOD on 2D PS@AuNP arrays maintained a highly improved operational stability compared to free GOD. The mechanism behind this effect is discussed. For practical applications, prepared PS@AuNP arrays can be used as an effective chip for GOD immobilization and application.
纳米/微米结构在纳米粒子的应用中起着重要作用。本文描述了一种在由聚苯乙烯（PS）制成的最大达约 1 cm²的大规模阵列上制造各种金纳米颗粒聚集体（AuNPs）的创新策略。树枝状表面活性剂 C18N3 具有多个胺基头，可以在 pH 值依赖的方式下控制吸附在 PS 球表面上的双层的厚度。通过控制 pH 值和在 C18N3 溶液中的浸渍时间，可以调节沉积在 PS 球上的 AuNPs（PS@AuNP）的形态。研究了纳米/微米结构对葡萄糖氧化酶（GOD）活性增强的影响。结果表明，与游离 GOD 和固定在 PS 阵列上的 GOD 相比，有序 PS@AuNP 阵列在 GOD 的比活性增强方面表现更好。此外，观察到固定在二维 PS@AuNP 阵列上的 GOD 与游离 GOD 相比，其操作稳定性得到了极大的提高。讨论了这种效应背后的机制。对于实际应用，制备的 PS@AuNP 阵列可用作 GOD 固定和应用的有效芯片。
