Laboratory for Polymer Analysis, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram 695012, India.
Anal Chem. 2011 Apr 1;83(7):2829-33. doi: 10.1021/ac1032879. Epub 2011 Mar 10.
Colloidal gold is extensively used for molecular sensing because of the flexibilities it offers in terms of modification of the gold nanoparticle surface with a variety of functional groups using thiol chemistry. We describe a simple assay that allows the visual detection of glucose in aqueous samples and demonstrates its applicability by estimating glucose in urine. To enable the glucose detection, we functionalized the thiol capped gold nanoparticles with glucose oxidase, the enzyme specific to β-D glucose, using carbodiimide chemistry. The visible color change of the GOD-functionalized gold nanoparticles from red to blue on interaction with glucose is the principle applied here for the sensing of urine glucose level. The solution turns blue when the glucose concentration exceeds 100 μg/mL. The approach depicted here seems to be important, particularly in third world countries where high tech diagnostics aids are inaccessible to the bulk of the population.
胶体金由于其在金纳米粒子表面用硫醇化学修饰各种官能团方面的灵活性，被广泛用于分子传感。我们描述了一种简单的测定法，允许在水溶液样品中进行葡萄糖的可视化检测，并通过估计尿液中的葡萄糖来证明其适用性。为了实现葡萄糖检测，我们使用碳二亚胺化学将葡萄糖氧化酶（特异性针对β-D 葡萄糖的酶）功能化到巯基封端的金纳米粒子上。在与葡萄糖相互作用时，GOD 功能化的金纳米粒子从红色变为蓝色，这是这里用于检测尿液葡萄糖水平的原理。当葡萄糖浓度超过 100μg/ml 时，溶液变为蓝色。这里描述的方法似乎很重要，特别是在高科技诊断辅助工具无法普及到大部分人口的第三世界国家。
