Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, Germany.
Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie, Charité - Universitätsmedizin Berlin, Germany.
Biosens Bioelectron. 2012 May 15;35(1):503-506. doi: 10.1016/j.bios.2012.03.024. Epub 2012 Mar 21.
This work describes the application of an impedance-based measurement for the real time evaluation of targeted tumor therapies in cell culture (HeLa cells). We used a treatment procedure that is well established in cells and mice. Therein, tumor cells are treated with a combination of an epidermal growth factor-based targeted toxin named SE and particular plant glycosides called saponins. In the present study HeLa cells were seeded in different numbers onto interdigitated electrode structures integrated into the bottom of a 96 well plate. The cells were treated with SE in the presence and absence of the saponin SpnS-1 (isolated from Saponaria officinalis roots). The impedance was directly correlated with the viability of the cells. As expected from known end point measurements, a concentration dependent enhancement of toxicity was observed; however, with the impedance measurement we were for the first time able to trace the temporal changes of cell death during the combination treatment. This substantially added to the understanding of initial cellular mechanisms in the augmentation of the toxicity of targeted toxins by saponins and indicated the superiority of real time monitoring over end point assays. The method is less labor intensive and label-free with ease of monitoring the effects at each time point.
本工作描述了一种基于阻抗的测量方法在细胞培养（HeLa 细胞）中实时评估靶向肿瘤治疗的应用。我们使用了一种在细胞和小鼠中已被充分证实的治疗程序。在此过程中，使用一种名为 SE 的基于表皮生长因子的靶向毒素和特定的植物糖苷（称为皂甙）联合处理肿瘤细胞。在本研究中，HeLa 细胞以不同的数量接种到集成在 96 孔板底部的叉指电极结构上。用 SE 处理细胞，同时存在和不存在皂甙 SpnS-1（从皂角根中分离得到）。阻抗与细胞活力直接相关。正如从已知终点测量中预期的那样，观察到了浓度依赖性的毒性增强；然而，通过阻抗测量，我们第一次能够在联合治疗期间追踪细胞死亡的时间变化。这大大增加了对皂甙增强靶向毒素毒性的初始细胞机制的理解，并表明实时监测优于终点测定。该方法劳动强度较低，无需标记，易于在每个时间点监测效果。
