Jerabek L, Gupta P, Ferrer J F
Cancer Res. 1979 Oct;39(10):3952-4.
This report describes the development of a 7-day infectivity assay for bovine leukemia virus (BLV) which is based on the induction of the major internal virion antigen (a protein with a molecular weight of 25,000) in susceptible indicator monolayer cell cultures. In this assay, the antigen is detected in the indicator cells by the immunoperoxidase antibody technique using a specific rabbit antiserum to a BLV protein with a molecular weight of 25,000. The immunoperoxidase infectivity assay is specific, quantitative, reproducible, and more sensitive than the previously described syncytia induction assay. The immunoperoxidase infectivity assay can be applied to the detection of BLV-infected cells and provides a reliable method for the direct diagnosis of BLV infection in cattle.
本报告描述了一种针对牛白血病病毒（BLV）的7天感染性检测方法的开发，该方法基于在易感指示单层细胞培养物中诱导主要内部病毒粒子抗原（一种分子量为25,000的蛋白质）。在该检测中，使用针对分子量为25,000的BLV蛋白的特异性兔抗血清，通过免疫过氧化物酶抗体技术在指示细胞中检测抗原。免疫过氧化物酶感染性检测具有特异性、定量性、可重复性，并且比先前描述的合胞体诱导检测更灵敏。免疫过氧化物酶感染性检测可用于检测BLV感染的细胞，并为牛BLV感染的直接诊断提供了一种可靠的方法。
