Walls A F, Jones D B, Williams J H, Church M K, Holgate S T
Immunopharmacology Group, University of Southampton, U.K.
J Pathol. 1990 Oct;162(2):119-26. doi: 10.1002/path.1711620204.
An avidin-biotin enhanced immunoperoxidase procedure using monoclonal antibodies (AA1, AA3, and AA5) prepared against human mast cell tryptase resulted in intense staining of mast cells in paraffin-embedded tissue. The distribution of mast cells observed was similar to that seen when adjacent serial sections were stained using a standard procedure with toluidine blue, though the immunoperoxidase technique permitted the identification of significantly more mast cells. With monoclonal antibody AA1, immunostaining was entirely specific for mast cell granules, and there was negligible background staining in a range of tissues including lung, tonsil, colon, gastric mucosa, skin, and pituitary. There was no staining of antibody on basophils or on any other normal blood leukocyte. The technique was effective with tissue fixed in either Carnoy's or neutral buffered formalin, though the internal mast cell structure was better preserved with formaldehyde fixation. The immunoperoxidase staining procedure with monoclonal antibody AA1 is a highly specific and sensitive means for the detection of mast cells in routinely processed tissues.
使用针对人肥大细胞类胰蛋白酶制备的单克隆抗体（AA1、AA3和AA5）的抗生物素蛋白-生物素增强免疫过氧化物酶程序，可使石蜡包埋组织中的肥大细胞产生强烈染色。观察到的肥大细胞分布与使用甲苯胺蓝标准程序对相邻连续切片进行染色时所见的分布相似，尽管免疫过氧化物酶技术能够识别出明显更多的肥大细胞。使用单克隆抗体AA1时，免疫染色对肥大细胞颗粒具有完全特异性，在包括肺、扁桃体、结肠、胃黏膜、皮肤和垂体在内的一系列组织中背景染色可忽略不计。嗜碱性粒细胞或任何其他正常血液白细胞上均无抗体染色。该技术对用卡诺氏固定液或中性缓冲福尔马林固定的组织均有效，不过用甲醛固定时肥大细胞的内部结构保存得更好。用单克隆抗体AA1进行的免疫过氧化物酶染色程序是检测常规处理组织中肥大细胞的一种高度特异性和灵敏的方法。
