Tong Xinxin, Chen Shengjie, Zheng Huanqin, Huang Shiguang, Lu Fangli
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.
Key Laboratory of Tropical Disease Control (Sun Yat-sen University), Ministry of Education, Guangzhou, Guangdong, 510080, China.
Parasitol Res. 2018 Jul;117(7):2255-2263. doi: 10.1007/s00436-018-5914-7. Epub 2018 May 19.
Interleukin 27 (IL-27) is a member of the IL-6/IL-12 family, and IL-27 receptor (IL-27R) consists of WSX-1 (the IL-27Rα subunit) and the signal-transducing subunit gp130. Human and mouse mast cells (MCs) express the IL-27R. To explore the expressions of IL-27/IL-27R subunits (WSX-1 and gp130) during acute ocular toxoplasmosis (OT), we established mouse model by intraocular injection of 500 Toxoplasma gondii RH strain tachyzoites. Histopathological changes were analyzed, MCs were counted by toluidine blue staining, and tryptase/IL-27 MCs were examined by immunofluorescence double-staining in the eyes and cervical lymph nodes (CLNs) of T. gondii-infected mice. The mRNA expressions of IL-27p28, WSX-1, gp130, and tachyzoite specific surface antigen 1 (SAG1) in the eyes and CLNs of T. gondii-infected mice, and the expressions of WSX-1 and gp130 in the murine mastocytoma cell line P815 infected with T. gondii tachyzoites in vitro were examined by using quantitative real-time reverse transcription-polymerase chain reaction. Our results showed that, after T. gondii infection, severe histopathological changes, increased numbers of total MCs and degranulated MCs, elevated expressions of IL-27p28, WSX-1, and gp130 were found in the eyes and CLNs, and significant correlations between the levels of IL-27 and SAG1 existed in the eyes and CLNs of T. gondii-infected mice. In addition, increased levels of WSX-1 and gp130 were examined in T. gondii-infected P815 cells. Our data suggested that IL-27/IL-27R expression induced by T. gondii infection may regulate MC-mediated immune response during acute OT in mouse model.
白细胞介素27（IL-27）是IL-6/IL-12家族的成员，白细胞介素27受体（IL-27R）由WSX-1（IL-27Rα亚基）和信号转导亚基gp130组成。人和小鼠肥大细胞（MCs）表达IL-27R。为了探究急性眼弓形虫病（OT）期间IL-27/IL-27R亚基（WSX-1和gp130）的表达情况，我们通过眼内注射500个刚地弓形虫RH株速殖子建立了小鼠模型。分析组织病理学变化，用甲苯胺蓝染色计数MCs，并通过免疫荧光双染色检测刚地弓形虫感染小鼠的眼睛和颈部淋巴结（CLNs）中的色氨酸酶/IL-27 MCs。采用定量实时逆转录-聚合酶链反应检测刚地弓形虫感染小鼠的眼睛和CLNs中IL-27p28、WSX-1、gp130和速殖子特异性表面抗原1（SAG1）的mRNA表达，以及体外感染刚地弓形虫速殖子的小鼠肥大细胞瘤细胞系P815中WSX-1和gp130的表达。我们的结果表明，刚地弓形虫感染后，在眼睛和CLNs中发现了严重的组织病理学变化、总MCs和脱颗粒MCs数量增加、IL-27p28、WSX-1和gp130表达升高，并且在刚地弓形虫感染小鼠的眼睛和CLNs中IL-27水平与SAG1水平之间存在显著相关性。此外，在刚地弓形虫感染的P815细胞中检测到WSX-1和gp130水平升高。我们的数据表明，刚地弓形虫感染诱导的IL-27/IL-27R表达可能在小鼠模型急性OT期间调节MC介导的免疫反应。
