Sashima M, Satoh M, Suzuki A
Department of Oral Pathology, School of Dentistry, Iwate Medical University, Morioka, Japan.
J Oral Pathol Med. 1990 Sep;19(8):381-4. doi: 10.1111/j.1600-0714.1990.tb00864.x.
Oral senile amyloidosis in senescence accelerated mouse (SAM) was examined for two SAM sublines (P/2/Iw and R/1/Iw) and for various ages by light microscopy, immunohistochemistry, and electron microscopy. The amyloid deposition, identified by green birefrigence following Congo red stain, was observed only in P/2/Iw. In P/2/Iw, no amyloid deposition was found at age 6 months; however, frequency and extent of such deposits increased with advancing age. Distribution of amyloid deposition was as follows: along papillary layers of mucous epithelium in the tongue, the gingiva, the palate, and the buccal mucosa; foci in connective tissues; along vessels, muscles, and minor salivary glands. Immunohistochemically, the amyloid deposition was positive with anti-ASSAM serum being raised against a unique amyloid protein ASSAM which probably induced "senile systemic amyloidosis". P/2/Iw is useful as an animal model of oral senile amyloidosis.
通过光学显微镜、免疫组织化学和电子显微镜，对两种衰老加速小鼠（SAM）亚系（P/2/Iw和R/1/Iw）以及不同年龄阶段的小鼠进行了口腔老年性淀粉样变性的研究。刚果红染色后呈现绿色双折射所鉴定的淀粉样沉积，仅在P/2/Iw中观察到。在P/2/Iw中，6个月龄时未发现淀粉样沉积；然而，随着年龄的增长，此类沉积物的频率和范围增加。淀粉样沉积的分布如下：沿舌、牙龈、腭和颊黏膜的黏液上皮乳头层；结缔组织中的病灶；沿血管、肌肉和小唾液腺。免疫组织化学显示，淀粉样沉积对抗ASSAM血清呈阳性反应，该血清是针对一种可能引发“老年性系统性淀粉样变性”的独特淀粉样蛋白ASSAM产生的。P/2/Iw可作为口腔老年性淀粉样变性的动物模型。
