Power M E, Olson M E, Domingue P A, Costerton J W
Department of Biological Sciences, University of Calgary, Alberta, Canada.
J Med Microbiol. 1990 Nov;33(3):189-98. doi: 10.1099/00222615-33-3-189.
Chronic osteomyelitis was produced by inoculating Staphylococcus aureus into rat tibia. The infection was characterised grossly by bone deformation and histopathologically by inflammation and the presence of coccal organisms sequestered within the bone tissue. Further observations by scanning electronmicroscopy demonstrated bacteria in microcolonies surrounded by dehydrated amorphous material that was considered to be glycocalyx. Transmission electronmicroscopy, when aided by antibody stabilisation, revealed extensive glycocalyx production within the tibia. These findings indicate that the rat model of chronic S. aureus osteomyelitis mimics the human infection with respect to the sessile mode of growth of bacteria within the bone. Serum antibody levels were assayed by ELISA and immunoblotting procedures. After an initial increase, ELISA titres remained relatively stable, apparently indicating the establishment of chronic osteomyelitis, whereas in immunoblotting an increase in titre over the course of infection was observed. Whole-cell ELISA revealed less subtle differences in antibody titre than did immunoblotting with cell-wall antigen. We found that mid-range antigens, including an antigen implicated as protein A, featured prominently in the immune response in this model of infection.
通过将金黄色葡萄球菌接种到大鼠胫骨中制造慢性骨髓炎。感染的大体特征是骨变形，组织病理学特征是炎症以及骨组织内存在被隔离的球菌。扫描电子显微镜的进一步观察显示，细菌存在于被认为是糖萼的脱水无定形物质包围的微菌落中。借助抗体稳定化的透射电子显微镜显示，胫骨内有大量糖萼产生。这些发现表明，慢性金黄色葡萄球菌骨髓炎的大鼠模型在细菌在骨内的固着生长模式方面模拟了人类感染。通过酶联免疫吸附测定（ELISA）和免疫印迹程序检测血清抗体水平。在最初升高后，ELISA效价保持相对稳定，这显然表明慢性骨髓炎已形成，而在免疫印迹中，观察到感染过程中效价升高。全细胞ELISA显示的抗体效价差异不如细胞壁抗原免疫印迹明显。我们发现，包括一种被认为是蛋白A的抗原在内的中等范围抗原在该感染模型的免疫反应中占主导地位。
