Department of Biophysics, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Ultrasound Med Biol. 2012 Jul;38(7):1205-11. doi: 10.1016/j.ultrasmedbio.2012.02.006. Epub 2012 Apr 13.
Sonodynamic therapy, an effect of low-power ultrasound field and the anticancer drug cisplatin, was studied in vitro on human melanoma cells A375. The viability of cells has been studied by standard 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide viability assay according to different modes of treatment: application of cisplatin alone, exposure of ultrasound field alone, exposure to ultrasound followed by cisplatin and application of cisplatin followed by exposure to ultrasound. Ultrasound was used at a therapeutic intensity of 1 W∙cm(-2) for 10 min. Concentration of cisplatin in the cell suspension was always 2.3 μM. The results show that sonodynamic therapy is one of the possibilities of how to intensify standard cytostatic therapy. This conclusion is supported by reducing the viability of studied cells, especially 72 h after the treatment. The time sequence of application of ultrasonic field and cytostatics appears to be a significant factor affecting the changes in cell viability. Maximum suppression of viability has been found when applying the experimental design involving application of cisplatin followed by exposure to ultrasound; the final value of viability of combined affected cells was more than 10% lower than for cisplatin treatment alone.
声动力学疗法是一种低强度超声场与抗癌药物顺铂联合作用的治疗方法，本研究在体外作用于人黑色素瘤 A375 细胞。根据不同的治疗方式，采用标准的 3-(4,5-二甲基噻唑-2-Yl)-2,5-二苯基四氮唑溴盐比色法来检测细胞活力：单独应用顺铂、单独暴露于超声场、超声暴露后应用顺铂以及顺铂处理后再暴露于超声。超声治疗强度为 1 W∙cm(-2)，持续 10 min。细胞悬浮液中顺铂的浓度始终为 2.3 μM。结果表明，声动力学疗法是增强标准细胞毒性疗法的可能性之一。这一结论得到了证实，即研究细胞活力降低，尤其是在治疗后 72 小时。应用超声场和细胞毒性药物的时间顺序似乎是影响细胞活力变化的一个重要因素。当采用顺铂处理后再暴露于超声的实验设计时，细胞活力的抑制达到最大值；联合作用的细胞活力最终值比单独应用顺铂处理时降低了 10%以上。
