Rezaei Masumeh, Esmailzadeh Arman, Shanei Ahmad
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
J Biomed Phys Eng. 2023 Oct 1;13(5):433-442. doi: 10.31661/jbpe.v0i0.2105-1337. eCollection 2023 Oct.
In the bystander effect, non-irradiated cells receive biological signals from adjacent irradiated cells and undergo a variety of alterations, considered recently in non-ionizing irradiation like ultrasound waves. In this study, the bystander effect of therapeutic ultrasound exposure alone and in combination with cisplatin was determined.
This study aims to determine the bystander effect caused by ultrasound and cisplatin.
This experimental study was conducted on the human melanoma cell line including two groups of target and bystander cells. The target cell group was divided into three sub-groups of ultrasound irradiation alone, cisplatin alone, and ultrasound irradiation in the presence of cisplatin that the culture medium of these three groups of cells was transferred to the bystander cell group using the medium transfer technique. Then, apoptotic bystander cells and the expression of P53 and HO-1 in target and bystander groups were measured.
The results of the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and apoptosis assay showed that cell death in target and bystander groups receiving the ultrasound with cisplatin is higher than in the ultrasound without cisplatin. PCR (the polymerase chain reaction) results in the target and bystander groups receiving treatments with increased expression of the P53 gene. Target and bystander groups receiving the ultrasound without cisplatin showed a decrease in HO-1 gene expression, while the ultrasound with cisplatin showed an increase in the HO-1 gene compared to the control group.
Combining ultrasound with ultrasound and without it can transfer bystander signals to the cells that are not directly treated.
在旁观者效应中，未受辐射的细胞从相邻的受辐射细胞接收生物信号并发生各种改变，最近在诸如超声波等非电离辐射中也有相关研究。在本研究中，确定了单纯治疗性超声照射以及超声与顺铂联合照射的旁观者效应。
本研究旨在确定超声和顺铂引起的旁观者效应。
本实验研究在人黑色素瘤细胞系上进行，包括靶细胞和旁观者细胞两组。靶细胞组分为单独超声照射、单独顺铂处理、超声与顺铂联合照射三个亚组，采用培养基转移技术将这三组细胞的培养基转移至旁观者细胞组。然后，检测旁观者细胞的凋亡情况以及靶细胞组和旁观者细胞组中P53和HO-1的表达。
MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐）和凋亡检测结果表明，接受超声与顺铂联合处理的靶细胞组和旁观者细胞组的细胞死亡高于接受单纯超声处理的组。靶细胞组和旁观者细胞组接受处理后的PCR（聚合酶链反应）结果显示P53基因表达增加。接受单纯超声处理的靶细胞组和旁观者细胞组HO-1基因表达降低，而与对照组相比，超声与顺铂联合处理组HO-1基因表达增加。
超声联合或不联合顺铂都可将旁观者信号传递至未直接处理的细胞。
