Chinese Academy of Sciences, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.
Bioresour Technol. 2012 Jun;114:561-6. doi: 10.1016/j.biortech.2012.02.119. Epub 2012 Mar 4.
This study devised a pathway in Escherichia coli for direct production of long chain hydroxyfatty acids (HFAs) from glucose. This is first report on the biosynthesis of HFAs from renewable sugar, without the need of exogenous fatty acids. By employing thioesterases BTE and 'TesA to tailor the composition of free fatty acids (FFAs) and using fatty acid hydroxylase P450(BM3) to convert FFAs to HFAs, high-specificity production of C12 and C14 HFAs was achieved. By further knocking out the endogenous fadD gene of E. coli, an engineered strain capable of producing 117.0 mg/L HFAs was finally obtained, representing a high HFA production in shake flask. This study indicated an attractive metabolic strategy for the biosynthesis of HFAs directly from renewable carbohydrates resources.
本研究在大肠杆菌中设计了一条直接利用葡萄糖生产长链羟基脂肪酸（HFAs）的途径。这是首次在不依赖外源脂肪酸的情况下，利用可再生糖来生物合成 HFAs 的报道。通过使用硫酯酶 BTE 和 'TesA 来调整游离脂肪酸（FFAs）的组成，并利用脂肪酸羟化酶 P450（BM3）将 FFAs 转化为 HFAs，实现了 C12 和 C14 HFAs 的高特异性生产。通过进一步敲除大肠杆菌内源 fadD 基因，最终获得了能够生产 117.0mg/L HFAs 的工程菌株，这代表了在摇瓶中 HFAs 的高产量。本研究为直接利用可再生碳水化合物资源生物合成 HFAs 提供了一种有吸引力的代谢策略。
