MUHC Reproductive Center, Div. of Reproductive Endocrinology and Infertility, Dept. of Obstetrics and Gynecology, McGill University, Montreal, Canada H3A 1A1.
Reprod Biomed Online. 2012 Jun;24(6):614-20. doi: 10.1016/j.rbmo.2012.02.009. Epub 2012 Feb 25.
This study retrospectively analysed array comparative genomic hybridization (CGH) results of 7753 embryos from 990 patients to determine the frequency of embryonic euploidy and its relationship with the cohort size (i.e. the number of embryos available for biopsy and array CGH analysis). Linear regression analysis was performed to assess the effect of cohort size on euploidy rate adjusted for the effect of female age. While increasing female age was associated with a significant decrease in euploidy rate of day-3 and day-5 embryos (P<0.001 for both groups), cohort size was not significantly associated with euploidy rate. Logistic regression analysis was performed to assess the effect of cohort size, adjusted for maternal age, on the likelihood of having at least one euploid embryo available for transfer. The odds of having at least one euploid embryo in an assisted cycle was significantly decreased by increasing female age (P<0.01 for both day-3 and day-5 embryos) and was significantly increased by every additional embryo available for analysis (P<0.001 for both day-3 and day-5 embryos).
本研究回顾性分析了 990 名患者的 7753 个胚胎的 array 比较基因组杂交 (CGH) 结果，以确定胚胎整倍体的频率及其与队列大小（即可供活检和 array CGH 分析的胚胎数量）的关系。线性回归分析用于评估队列大小对女性年龄影响调整后的整倍体率的影响。虽然女性年龄的增加与第 3 天和第 5 天胚胎的整倍体率显著降低相关（两组均 P<0.001），但队列大小与整倍体率无显著相关性。逻辑回归分析用于评估队列大小（按母体年龄调整）对至少有一个可用于转移的整倍体胚胎的可能性的影响。辅助周期中至少有一个整倍体胚胎的可能性随着女性年龄的增加而显著降低（第 3 天和第 5 天胚胎均 P<0.01），并且随着可供分析的胚胎数量的增加而显著增加（第 3 天和第 5 天胚胎均 P<0.001）。
