State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
J Microbiol Methods. 2012 Jun;89(3):153-8. doi: 10.1016/j.mimet.2012.03.018. Epub 2012 Apr 3.
The purpose of this study was to determine the interactions between arsenic (As) resistant bacteria and As, using surface-enhanced Raman scattering (SERS) and Fourier transform infrared (FTIR) spectroscopy. According to our 16S rDNA results, eight bacteria isolated from the environment can be identified to four genera (Arthrobacter, Pseudomonas, Sphingomonas, and Acinetobacter). The bacteria were separated into cell wall and protoplast in the study to assess the As(V) attack. The As(V) stress on bacteria could be identified with SERS, but not with FTIR. The bacteria in our study primarily resist As(V) through sequestration of As(V) by the cell wall. The change in SERS peaks and their relationships with cell wall suggested that As(V) mainly interacts with functional groups on the cell wall including polysaccharides and flavin derivates.
本研究旨在使用表面增强拉曼散射（SERS）和傅里叶变换红外（FTIR）光谱法来确定砷（As）抗性细菌与 As 之间的相互作用。根据我们的 16S rDNA 结果，从环境中分离出的 8 种细菌可鉴定为 4 个属（节杆菌、假单胞菌、鞘氨醇单胞菌和不动杆菌）。在这项研究中，细菌被分离成细胞壁和原生质体，以评估 As(V)的攻击。SERS 可以识别 As(V)对细菌的胁迫，但 FTIR 则不行。本研究中的细菌主要通过细胞壁螯合 As(V)来抵抗 As(V)。SERS 峰的变化及其与细胞壁的关系表明，As(V)主要与细胞壁上的功能基团相互作用，包括多糖和黄素衍生物。
