State Key Laboratory of Food Science and Technology, Jiangnan University, Jiangsu Province, China.
School of Food Science and Technology, Jiangnan University, Jiangsu Province, China.
Sci Rep. 2019 Jan 24;9(1):490. doi: 10.1038/s41598-018-36989-7.
The antifungal mechanism of essential oils against fungi remains in the shallow study. In this paper, antifungal mechanism of trans-cinnamaldehyde against Penicillium italicum was explored. Trans-cinnamaldehyde exhibited strong mycelial growth inhibition against Penicillium italicum, with minimum inhibitory concentration of 0.313 μg/mL. Conventional analytical tests showed that trans-cinnamaldehyde changed the cell membrane permeability, which led to the leakage of some materials. Meanwhile, the membrane integrity and cell wall integrity also changed. Surface-enhanced Raman spectroscopy, an ultrasensitive and fingerprint method, was served as a bran-new method to study the antifungal mechanism. Characteristic peaks of supernatant obviously changed at 734, 1244, 1330, 1338 and 1466 cm. The Raman intensity represented a strong correlation with results from conventional methods, which made SERS an alternative to study antifungal process. All evidences implied that trans-cinnamaldehyde exerts its antifungal capacity against Penicillium italicum via membrane damage mechanism.
精油对真菌的抗真菌机制仍处于浅层研究阶段。本文探讨了肉桂醛对意大利青霉的抗真菌机制。肉桂醛对意大利青霉的菌丝生长有很强的抑制作用，最小抑菌浓度为 0.313μg/mL。常规分析测试表明，肉桂醛改变了细胞膜的通透性，导致一些物质泄漏。同时，膜的完整性和细胞壁的完整性也发生了变化。表面增强拉曼光谱作为一种超灵敏和指纹方法，被用作研究抗真菌机制的全新方法。上清液的特征峰在 734、1244、1330、1338 和 1466cm 处明显改变。拉曼强度与常规方法的结果呈很强的相关性，这使得 SERS 成为研究抗真菌过程的一种替代方法。所有证据表明，肉桂醛通过破坏细胞膜的机制发挥其对意大利青霉的抗真菌能力。
