Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedics, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, People's Republic of China.
Biomaterials. 2012 Jul;33(20):5076-84. doi: 10.1016/j.biomaterials.2012.03.069. Epub 2012 Apr 13.
Bone defects are common in elderly patients suffering from osteoporosis. Current methods of bone defect treatment for osteoporosis are not always satisfactory. In this study, we demonstrated that bone marrow mesenchymal stem cells (MSCs) harvested from goats with long-term estrogen deficiencies exhibited a lower proliferation rate and decreased osteogenic capacity, which are critical obstacles for bone defect repair in the elderly. However, by combining autologous enriched bone marrow mesenchymal stem cells with porous β-TCP, we successfully repaired critical-sized bone defects in the medial femoral condyle of the osteoporotic goats. Both micro-CT images and histomorphometry analysis illustrated improved bone formation following the enriched MSC therapy. Thus, we proposed autologous enriched bone marrow mesenchymal stem cells as a quick, safe therapeutic strategy to treat osteoporotic bone defects.
骨缺损在老年骨质疏松症患者中很常见。目前治疗骨质疏松症骨缺损的方法并不总是令人满意。在这项研究中，我们证明了从长期雌激素缺乏的山羊中提取的骨髓间充质干细胞（MSCs）增殖率较低，成骨能力下降，这是老年患者骨缺损修复的关键障碍。然而，通过将自体富集中的骨髓间充质干细胞与多孔β-TCP 结合，我们成功地修复了骨质疏松症山羊股骨内侧髁的临界尺寸骨缺损。微 CT 图像和组织形态计量学分析表明，富集中的 MSC 治疗后骨形成得到改善。因此，我们提出自体富集中的骨髓间充质干细胞是一种快速、安全的治疗策略，可用于治疗骨质疏松性骨缺损。
