School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.
Acta Biomater. 2012 Jul;8(7):2697-703. doi: 10.1016/j.actbio.2012.04.010. Epub 2012 Apr 10.
Materials that adhere to the endothelial cell (EC) lining of blood vessels may be useful for treating vascular injury. Cell adhesion molecules (CAMs), such as endothelial leukocyte adhesion molecule-1 (E-selectin) and vascular cell adhesion molecule-1 (VCAM1), modulate EC-leukocyte interactions. In this study, we mimicked cell-cell interactions by seeding cells on alginate hydrogels modified with antibodies against E-selectin and VCAM1, which are upregulated during inflammation. ECs were activated with interleukin-1α to increase CAM expression and subsequently seeded onto hydrogels. The strength of cell adhesion onto gels was assessed via a centrifugation assay. Strong, cooperative EC adhesion was observed on hydrogels presenting a 1:1 ratio of anti-VCAM1:anti-E-selectin. Cell adhesion was stronger on dual functionalized gels than on gels modified with anti-VCAM1, anti-E-selectin or the arginine-glycine-aspartic acid (RGD) peptide alone. Anti-VCAM1:anti-E-selectin-modified hydrogels may be engineered to adhere the endothelium cooperatively.
材料黏附在血管内皮细胞（EC）衬里上可能有助于治疗血管损伤。细胞黏附分子（CAM），如内皮白细胞黏附分子-1（E-选择素）和血管细胞黏附分子-1（VCAM1），调节 EC-白细胞相互作用。在这项研究中，我们通过将细胞接种在经过针对 E-选择素和 VCAM1 的抗体修饰的藻酸盐水凝胶上来模拟细胞-细胞相互作用，这些抗体在炎症期间上调。用白细胞介素-1α激活 EC 以增加 CAM 的表达，然后将其接种到水凝胶上。通过离心试验评估细胞在凝胶上的黏附强度。在呈现抗 VCAM1：抗 E-选择素 1:1 比例的水凝胶上观察到强烈的协同 EC 黏附。与单独用抗 VCAM1、抗 E-选择素或精氨酸-甘氨酸-天冬氨酸（RGD）肽修饰的凝胶相比，双功能化凝胶上的细胞黏附更强。抗 VCAM1：抗 E-选择素修饰的水凝胶可被设计为协同黏附内皮细胞。
