Hubei Key Laboratory of Genetic Regulation and Integrated Biology, College of Life Sciences, Central China Normal University, Wuhan, Hubei 430079, China.
Comp Biochem Physiol A Mol Integr Physiol. 2012 Aug;162(4):303-9. doi: 10.1016/j.cbpa.2012.03.021. Epub 2012 Apr 5.
Fish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rarus is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rarus dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rarus.
鱼类的性别决定和分化具有多样性。DMRT1 和芳香酶在门水平上是保守的，在性别发育中发挥关键作用。拉氏小脂鲤是中国水生毒理学研究中的一种小型鱼类模型，用于研究环境内分泌干扰物对基因表达的影响，但它的性发育仍然难以捉摸。在这里，我们报道了拉氏小脂鲤 dmrt1 的全长 cDNA 及其在成年期和个体发生过程中的表达情况，以及编码性腺和脑型芳香酶的两个基因 cyp19a1a 和 cyp19a1b 的表达情况。cyp19a1a 和 dmrt1 都在卵巢和睾丸中表达，但表现出性别二态性。cyp19a1a 在卵巢中的表达高于睾丸，而 dmrt1 的表达则相反。孵化后 15 天，幼鱼性腺组织学发生变化。dmrt1 和 cyp19a1a 的二态性表达从孵化后 5 天开始出现，早于组织学变化。cyp19a1b 与 cyp19a1a 协调表达，直到孵化后 15 天。这些结果表明，dmrt1 和 cyp19a1a 在拉氏小脂鲤的性别决定和性别分化中发挥重要作用。
