Dong Junjian, Li Jia, Hu Jie, Sun Chengfei, Tian Yuanyuan, Li Wuhui, Yan Ningning, Sun Chengxi, Sheng Xihui, Yang Song, Shi Qiong, Ye Xing
Key Laboratory of Tropical and Subtropical Fisheries Resources Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Shenzhen Key Lab of Marine Genomics, Guangdong Provincial Key Lab of Molecular Breeding in Marine Economic Animals, BGI Academy of Marine Sciences, BGI Marine, BGI Group, Shenzhen, China.
Front Genet. 2020 Nov 12;11:563947. doi: 10.3389/fgene.2020.563947. eCollection 2020.
Doublesex and mab-3-related transcription factor () genes are widely distributed across various biological groups and play critical roles in sex determination and neural development. Here, we applied bioinformatics methods to exam cross-species changes in the dmrt family members and evolutionary relationships of the genes based on genomes of 17 fish species. All the examined fish species have while only five species contained Most fish harbored two paralogs ( and ), with being unique to fish. In the phylogenetic tree, 147 DMRT are categorized into eight groups (DMRT1-DMRT8) and then clustered in three main groups. Selective evolutionary pressure analysis indicated purifying selections on genes and the gene cluster. Similar genomic conservation patterns of the gene cluster with 20-kb upstream/downstream regions in fish with various sex-determination systems were observed except for three regions with remarkable diversity. Synteny analysis revealed that , and were relatively conserved in fish during the evolutionary process. While was lost in most species during evolution. The high conservation of the gene cluster in various fish genomes suggests their crucial biological functions while various family members and sequences across fish species suggest different biological roles during evolution. This study provides a molecular basis for fish functional analysis and may serve as a reference for in-depth phylogenomics.
双性和mab - 3相关转录因子（doublesex and mab-3-related transcription factor，DMRT）基因广泛分布于各种生物群体中，在性别决定和神经发育中发挥着关键作用。在此，我们应用生物信息学方法，基于17种鱼类的基因组，研究DMRT家族成员的跨物种变化以及DMRT基因的进化关系。所有被检测的鱼类物种都有DMRT基因，而只有5个物种含有DMRT2基因。大多数鱼类含有两个DMRT1旁系同源基因（DMRT1a和DMRT1b），DMRT2是鱼类特有的。在系统发育树中，147个DMRT被分为8个组（DMRT1 - DMRT8），然后聚为三个主要类群。选择性进化压力分析表明，对DMRT基因和DMRT基因簇存在纯化选择。除了三个具有显著多样性的区域外，在具有不同性别决定系统的鱼类中，观察到DMRT基因簇及其上下游20 kb区域具有相似的基因组保守模式。共线性分析表明，在进化过程中，DMRT1、DMRT2和DMRT3在鱼类中相对保守。而DMRT4在大多数物种进化过程中丢失。DMRT基因簇在各种鱼类基因组中的高度保守表明它们具有关键的生物学功能，而不同鱼类物种中的各种DMRT家族成员和序列表明它们在进化过程中具有不同的生物学作用。本研究为鱼类DMRT功能分析提供了分子基础，并可为深入的系统发育基因组学研究提供参考。
