Mailman D, Rose C
Biology Department, University of Houston, TX 77204.
Life Sci. 1990;47(19):1737-44. doi: 10.1016/0024-3205(90)90347-t.
The possibility that significant amounts of fatty acids were dissolved in or bound to the surfaces of common laboratory materials was examined. The uptake or adsorption of radioisotopically labeled oleic acid and cholic acid by plastic tubing of Tygon, Teflon, and polyethylene, and Pyrex, and borosilicate glass, and steel was measured. 3H-oleic acid and 14C-cholic acid were used in the presence of different concentrations of unlabeled oleic acid, cholic acid, and/or bovine serum albumin. Concentrations, composition, pH, and perfusion rates were varied. Relatively large amounts (10-95%) of oleic acid (25 microM) were lost by dissolving in plastic and adsorption to glass or metal. The degree of losses decreased in the presence of compounds in the perfusion solution which could bind or dissolve oleic acid. In contrast, cholic acid was not lost to plastic, glass or metal. The magnitude of and influence of perfusion rate, composition, pH, and sequence of perfusion solutions on oleic acid losses were sufficiently large that the results of certain studies, such as those of unstirred water layers of albumin - stimulated fatty acid uptake by hepatocytes may need to be reexamined.
研究了大量脂肪酸溶解于或结合在常见实验室材料表面的可能性。测定了泰贡（Tygon）、聚四氟乙烯（Teflon）、聚乙烯、派热克斯（Pyrex）、硼硅酸盐玻璃和钢等材质的塑料管对放射性同位素标记的油酸和胆酸的摄取或吸附情况。在不同浓度的未标记油酸、胆酸和/或牛血清白蛋白存在的情况下，使用了³H - 油酸和¹⁴C - 胆酸。改变了浓度、成分、pH值和灌注速率。相对大量（10 - 95%）的油酸（25微摩尔）因溶解于塑料以及吸附到玻璃或金属上而损失。在灌注溶液中存在能够结合或溶解油酸的化合物时，损失程度降低。相比之下，胆酸不会损失到塑料、玻璃或金属上。灌注速率、成分、pH值以及灌注溶液顺序对油酸损失的影响程度足够大，以至于某些研究的结果，例如那些关于白蛋白刺激肝细胞摄取脂肪酸的未搅拌水层研究结果，可能需要重新审视。
