Zapata Paola, Rojas Diego, Atehortúa Lucía
Biotechnology Group-Universidad de Antioquia, Medellin, Colombia.
Int J Med Mushrooms. 2012;14(2):197-203. doi: 10.1615/intjmedmushr.v14.i2.80.
The effect of different non-conventional carbon sources was studied in the submerged culture of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, for simultaneous production of mycelial biomass, bioactive ganoderic acid, and polysaccharides, in less time, using non-conventional carbon sources to minimize the high costs of current culture media. The optimal medium composition was defined as (g/L): 50 of barley flour, 0.2 of KH2PO4, 0.1 of MgSO4ⁱ7H2O, and 1 NH4Cl. Cultivated under this complex culture medium, the mycelial biomass production was 23.49 ± 0.37 g/L; the extracellular polysaccharides production was 2.72 ± 0.11 g/L; the intracellular polysaccharides production was 2.22 ± 0.06 g/L; the ganoderic acids production was 299.67 ± 11.63 mg/L. One liter of culture medium developed in this project was priced at USD $ 0.11 if barley flour is used as carbon source or $ 0.13 with oat flour in order to get a good amount of products of interest.
研究了不同非常规碳源对灵芝（Ganoderma lucidum）深层培养的影响，旨在利用非常规碳源在更短时间内同时生产菌丝体生物量、生物活性灵芝酸和多糖，以尽量降低当前培养基的高成本。最佳培养基组成确定为（g/L）：大麦粉50、KH₂PO₄ 0.2、MgSO₄·7H₂O 0.1和NH₄Cl 1。在这种复合培养基下培养，菌丝体生物量产量为23.49±0.37 g/L；胞外多糖产量为2.72±0.11 g/L；胞内多糖产量为2.22±0.06 g/L；灵芝酸产量为299.67±11.63 mg/L。如果使用大麦粉作为碳源，本项目开发的一升培养基价格为0.11美元；若使用燕麦粉则为0.13美元，以便获得大量目标产物。
