School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, 53706, USA.
Foodborne Pathog Dis. 2012 May;9(5):480-6. doi: 10.1089/fpd.2011.1013. Epub 2012 Apr 16.
In this study, we demonstrate that purA and purB transposon mutants of serotype 4b Listeria monocytogenes were severely impaired in their ability to colonize the gastrointestinal tract and cause systemic infection of the spleen, liver, and gallbladder following intragastric inoculation of A/J mice. The mutant strains were also impaired in their ability to multiply within Caco-2 human intestinal epithelial cells. Neither mutant was affected in resistance to synthetic gastric fluid (pH 4.5). These findings indicate that purine biosynthesis is critical for gastrointestinal virulence of L. monocytogenes serotype 4b in mice.
在这项研究中，我们证明了血清型 4b 李斯特菌 purA 和 purB 转座子突变体在经胃内接种 A/J 小鼠后，其在胃肠道定殖和引起脾脏、肝脏和胆囊全身感染的能力严重受损。这些突变株在 Caco-2 人肠道上皮细胞内的增殖能力也受到损害。两种突变株对合成胃液（pH 值 4.5）的抗性均不受影响。这些发现表明嘌呤生物合成对于血清型 4b 李斯特菌在小鼠中的胃肠道毒力至关重要。
