Department of Periodontics, S.R.M Dental College and Hospital, Chennai, Tamil Nadu, India.
Int J Immunopathol Pharmacol. 2012 Jan-Mar;25(1):127-34. doi: 10.1177/039463201202500115.
It has been established that human dental pulp and periodontal ligament contain a population of mesenchymal stem cells (MSCs). However, the phenotypic analysis in terms of putative stem cell markers expressed by these stem cell populations is incomplete. It is relevant to understand whether stem cells derived from closely related tissues are programmed differently. The aim of the present study is to analyze whether these stem cells depict distinct characteristics by gaining insight into differences in their immunophenotype. Dental pulp and periodontal ligament tissue samples were obtained from extracted impacted wisdom teeth. Cell cultures were analyzed for surface and intracellular markers by indirect immunoflourescence. Detailed immunophenotype analysis was carried out by flow cytometry using relevant markers. The present study data shows dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) expressed embryonic stem (ES) cell markers Oct-4, Nanog and mesodermal marker Vimentin by indirect immunoflourescence. PDLSCs, however, had a weak expression of Nanog. Immunophenotyping revealed strong expression of MSC markers (CD73, CD90) in DPSCs and PDLSCs. Differences were observed in expression of stemness-related markers. DPSCs displayed increased percentages of SSEA4, CD13 and CD166 and decreased CD9 expression compared to PDLSCs. Both stem cells express common MSC markers, different levels of expression suggests there might be more than one stem cell population existing within these tissues which differ in their embryonic status, and DPSCs are a more primitive stem cell population in comparison to PDLSCs.
已经证实，人类牙髓和牙周韧带中含有一群间充质干细胞（MSCs）。然而，这些干细胞群体所表达的假定干细胞标志物的表型分析并不完整。了解来源于密切相关组织的干细胞是否具有不同的编程方式是很重要的。本研究旨在通过深入了解其免疫表型的差异，分析这些干细胞是否具有不同的特征。从拔除的埋伏智齿中获取牙髓和牙周韧带组织样本。通过间接免疫荧光法分析细胞培养物的表面和细胞内标志物。通过使用相关标志物的流式细胞术进行详细的免疫表型分析。本研究数据显示，牙髓干细胞（DPSCs）和牙周韧带干细胞（PDLSCs）通过间接免疫荧光法表达胚胎干细胞（ES）标志物 Oct-4、Nanog 和中胚层标志物波形蛋白。然而，PDLSCs 中 Nanog 的表达较弱。免疫表型分析显示，DPSCs 和 PDLSCs 中 MSC 标志物（CD73、CD90）的表达较强。在干细胞相关标志物的表达上存在差异。DPSCs 显示出较高的 SSEA4、CD13 和 CD166 百分比，而 CD9 的表达则降低，与 PDLSCs 相比。这两种干细胞都表达常见的 MSC 标志物，不同的表达水平表明，这些组织中可能存在不止一种干细胞群体，它们在胚胎状态上存在差异，而与 PDLSCs 相比，DPSCs 是一种更为原始的干细胞群体。
