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犬牙周膜来源间充质干细胞的研究：牙周再生有前景工具的可能性。

investigation of canine periodontal ligament-derived mesenchymal stem cells: A possibility of promising tool for periodontal regeneration.

作者信息

Salari Sedigh Hamideh, Saffarpour Anna, Jamshidi Shahram, Ashouri Mahdi, Nassiri Seyed Mahdi, Dehghan Mohammad Mehdi, Ranjbar Esmail, Shafieian Reyhaneh

机构信息

Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.

Department of Periodontology, Tehran University of Medical Sciences, International Campus, Tehran, Iran.

出版信息

J Oral Biol Craniofac Res. 2023 May-Jun;13(3):403-411. doi: 10.1016/j.jobcr.2023.03.010. Epub 2023 Apr 18.

DOI:10.1016/j.jobcr.2023.03.010
PMID:37113531
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10127137/
Abstract

OBJECTIVES

Recent investigations indicate that canine periodontal ligament-derived stem cells (cPDLSCs) may reveal a reliable strategy for repair of periodontal tissues via cell-based tissue engineering approaches Due to limited research, his study aimed to demonstrate the phenotypic characterization of cPDLSc in comparison with canine bone marrow-derived mesenchymal stem cells (cBMSCs)

METHODS

Mesenchymal stem cells (MSCs) were obtained from PDL and BM of five male adult Mongrel dogs. isolation and expansion as well as biologic characterization including colony unit formation (CFU), osteogenic and adipogenic differentiation, flow cytometric analysis of CD34 and CD44, and RT-PCR of alkaline phosphatase (ALP), osteocalcin (OCN), periostin (POSTN) and S100A4 were performed. Furthermore, electron microscopy analysis was done to complement the comparative research.

RESULTS

CFU assay revealed that colonies of cPDLSCs presented 70% confluency with a more finite lifespan than BM-MSCs, showing a significant increase in cPDLSCs. Both types of MSCs showed osteogenic and adipogenic phenotypic characterized with clusters of mineralized depositions and lipid vacuoles, respectively. Both types of MSCs expressed CD44 with limited expression of CD34. RT-PCR of cPDLSCs revealed that expression of ALP, POSTN, OCN and S100A4 genes were significantly higher than those of BMSCs. In addition, comparison of SEM and revealed that cPDLSCs expressed more extracellular collagen fibers.

CONCLUSIONS

The current study indicated that cPDLSCs show potency as a novel cellular therapy for periodontal regeneration a large animal model.

摘要

目的

近期研究表明，犬牙周膜来源的干细胞（cPDLSCs）可能通过基于细胞的组织工程方法为牙周组织修复提供一种可靠策略。由于相关研究有限，本研究旨在对比犬骨髓来源的间充质干细胞（cBMSCs），阐明cPDLSCs的表型特征。

方法

从5只成年雄性杂种犬的牙周膜和骨髓中获取间充质干细胞（MSCs）。进行分离、扩增以及生物学特性鉴定，包括集落形成单位（CFU）、成骨和成脂分化、CD34和CD44的流式细胞术分析，以及碱性磷酸酶（ALP）、骨钙素（OCN）、骨膜蛋白（POSTN）和S100A4的逆转录聚合酶链反应（RT-PCR）。此外，进行电子显微镜分析以补充对比研究。

结果

CFU分析显示，cPDLSCs的集落达到70%汇合，其寿命比骨髓间充质干细胞更有限，cPDLSCs显著增加。两种类型的MSCs均表现出成骨和成脂表型特征，分别有矿化沉积簇和脂滴。两种类型的MSCs均表达CD44，CD34表达有限。cPDLSCs的RT-PCR显示，ALP、POSTN、OCN和S100A4基因的表达明显高于骨髓间充质干细胞。此外，扫描电子显微镜比较显示，cPDLSCs表达更多的细胞外胶原纤维。

结论

本研究表明，在大型动物模型中，cPDLSCs作为牙周再生的新型细胞疗法具有潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/15e292e0be2b/gr7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/86ad7c776b4b/gr3.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/d0f4ec349e85/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/bba6d168d05b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/15e292e0be2b/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/e872df92c97f/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/9ef505ef278f/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/413d061d2ecc/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/86ad7c776b4b/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/4b2a105913d8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/d0f4ec349e85/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/bba6d168d05b/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0212/10127137/15e292e0be2b/gr7.jpg

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