Murta J N, Andrade I C, Cunha-Vaz J G
Centro de Oftalmologia de Universidade de Coimbra, Portugal.
Ophtalmologie. 1990 Jan-Feb;4(1):28-9.
An experimental model was developed to study the permeability of individual retinal vessels in vitro using microperfusion techniques adapted from the kidney tubule studies. Inulin was used as the reference tracer and D-glucose as the test tracer. We found a concentration dependent flux of D-glucose from lumen to bath that increased as D-glucose concentrations in the lumen rose. The flattening of the hyperbolic curve at the higher D-glucose concentrations in the lumen appears consistent with the Michaelis-Menten equation (Km = 6 x 10(-3) M).
利用从肾小管研究中改编而来的微灌注技术，开发了一种实验模型，用于在体外研究单个视网膜血管的通透性。菊粉用作参考示踪剂，D-葡萄糖用作测试示踪剂。我们发现，随着管腔内D-葡萄糖浓度的升高，D-葡萄糖从管腔到浴液的通量呈浓度依赖性增加。在管腔内较高的D-葡萄糖浓度下，双曲线的扁平化似乎与米氏方程（Km = 6×10⁻³ M）一致。
