Zhu Xian-Feng, Qin Ren-Bing, Yu Chen-Chen, Fan Shu-Jun
Institute of Bioengineering, College of Life Science, Henan University, Kaifeng 475004, China.
Huan Jing Ke Xue. 2012 Feb;33(2):495-8.
Laccase extracted from the Amillariella mellea fermentation catalytic decolored on two common anthraquinone dyes: Reactive Brilliant Blue KN-R and Reactive Brilliant Blue X-BR which is broadly used in the printing and dyeing industry and obtained the optimal catalytic decolorizing conditions. The results showed that optimum temperature of Reactive Brilliant Blue KN-R decolorization was 30 degrees C, the optimum dye concentrations was 80 mg x L(-1), the optimum enzyme dosage was 0.25 U x mL(-1), and the optimum pH was 5. Under this optimal conditions, the maximum decolorization rate of Reactive Brilliant Blue KN-R was over 90%. The optimum temperature Reactive Brilliant Blue X-BR decolorization was 30 degrees C, the optimum dye concentrations was 50 mg x L(-1), the optimum enzyme dosage was 0.5 U x mL(-1), and the optimum was pH 4. Under the optimal conditions, the maximum decolorization rate of Reactive Brilliant Blue X-BR was over 70%. The decolorization on the two common industrial dyes by crude enzyme from Amillariella mellea fermentation obtained a good results. The results indicated that the decoloration on anthraquinone dyes by laccase from Amillariella mellea has a potential value in the printing and dyeing industry.
从蜜环菌发酵液中提取的漆酶对两种常见的蒽醌染料——活性艳蓝KN-R和活性艳蓝X-BR进行催化脱色。活性艳蓝X-BR广泛应用于印染工业，并获得了最佳催化脱色条件。结果表明，活性艳蓝KN-R脱色的最适温度为30℃，最佳染料浓度为80mg·L⁻¹，最佳酶用量为0.25U·mL⁻¹，最佳pH为5。在此最佳条件下，活性艳蓝KN-R的最大脱色率超过90%。活性艳蓝X-BR脱色的最适温度为30℃，最佳染料浓度为50mg·L⁻¹，最佳酶用量为0.5U·mL⁻¹，最佳pH为4。在最佳条件下，活性艳蓝X-BR的最大脱色率超过70%。蜜环菌发酵粗酶对两种常见工业染料的脱色取得了良好效果。结果表明，蜜环菌漆酶对蒽醌染料的脱色在印染工业中具有潜在价值。
