Institute of Drug Chemistry and Metabolism (IQUIMEFA) (UBA-CONICET), School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina.
Phytother Res. 2013 Feb;27(2):192-8. doi: 10.1002/ptr.4700. Epub 2012 Apr 18.
The fresh leaves and stems of Ilex paraguariensis (Aquifoliaceae) are employed to prepare the commercial product used in North-eastern Argentina, Southern Brazil and Eastern Paraguay named yerba maté. The presence of polyphenols and xanthines, which present antioxidant activity, has been described in I. paraguariensis. In living organism, reactive oxygen species can cause tissue damage affecting erythrocyte membranes leading to hemolysis. The aim of this work was to evaluate the protective effect of an aqueous extract of I. paraguariensis (green leaves) on the hemolysis of red blood cells induced by hydrogen peroxide and to correlate this activity with the enzymatic activity related to hydrogen peroxide metabolism. The antioxidant activity of chlorogenic acid and caffeine was also analysed to evaluate their contribution to the activity of the crude extract. The extract as well as the isolated compounds protected red blood cells from hemolysis. This effect was related to a catalase-like activity. This study could contribute to the knowledge of the antioxidant activity of I. paraguariensis in view of the great quantities of yerba maté consumed by the population.
冬青科的巴拉圭茶新鲜的叶子和茎被用来制备商业产品，在阿根廷东北部、巴西南部和巴拉圭东部被称为马黛茶。已在 I. paraguariensis 中描述了具有抗氧化活性的多酚和黄嘌呤的存在。在活生物体中，活性氧会导致组织损伤，影响红细胞膜，导致溶血。本工作的目的是评估来自 I. paraguariensis（绿叶）的水提取物对由过氧化氢诱导的红细胞溶血的保护作用，并将这种活性与与过氧化氢代谢相关的酶活性相关联。还分析了绿原酸和咖啡因的抗氧化活性，以评估它们对粗提物活性的贡献。提取物和分离的化合物都能保护红细胞不发生溶血。这种作用与过氧化氢酶样活性有关。鉴于大量的马黛茶被人群消费，这项研究可能有助于了解 I. paraguariensis 的抗氧化活性。
