通过电穿孔竞争 DNA 转染配方对人脂肪干细胞和间充质干细胞。
Competitive DNA transfection formulation via electroporation for human adipose stem cells and mesenchymal stem cells.
机构信息
Department of Pediatrics Research, The University Texas MD Anderson Cancer Center, Graduate School of Biomedical Sciences, 1515 Holcombe, Houston, TX, USA.
出版信息
Biol Proced Online. 2012 Apr 18;14(1):7. doi: 10.1186/1480-9222-14-7.
BACKGROUND
Adipose stem cells have a strong potential for use in cell-based therapy, but the current nucleofection technique, which relies on unknown buffers, prevents their use.
RESULTS
We developed an optimal nucleofection formulation for human adipose stem cells by using a three-step method that we had developed previously. This method was designed to determine the optimal formulation for nucleofection that was capable of meeting or surpassing the established commercial buffer (Amaxa), in particular for murine adipose stem cells. By using this same buffer, we determined that the same formulation yields optimal transfection efficiency in human mesenchymal stem cells.
CONCLUSIONS
Our findings suggest that transfection efficiency in human stem cells can be boosted with proper formulation.
背景
脂肪干细胞在基于细胞的治疗中有很强的应用潜力，但目前依赖于未知缓冲液的核转染技术阻止了它们的使用。
结果
我们使用先前开发的三步法为人类脂肪干细胞开发了最佳的核转染配方。该方法旨在确定最佳的核转染配方，以满足或超过既定的商业缓冲液（Amaxa），特别是对于鼠脂肪干细胞。使用相同的缓冲液，我们确定相同的配方可在人骨髓间充质干细胞中产生最佳的转染效率。
结论
我们的研究结果表明，通过适当的配方可以提高人干细胞的转染效率。
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