Department of Pediatric Surgery and Urology, AP-HP, Robert Debré University Hospital, University of Paris Diderot, Paris, France.
Urology. 2012 Aug;80(2):486.e1-7. doi: 10.1016/j.urology.2012.02.020. Epub 2012 Apr 17.
To evaluate the effect of the infection and the obstruction of urinary tract on renal parenchyma, we developed a model of ascending urinary infection in mice with partial unilateral obstructive uropathy.
Six week-old CBA/J mice were operated on to perform a right partial ureteral obstruction and challenged transurethrally with Escherichia coli CFT073 (high virulent strain) or E. coli Mez (low virulent strain). Level of infection of urine and kidneys, score of inflammation and fibrosis of kidneys, and kidney weight ratio (KWR) in the short and long term were studied.
At short-term (72 hours) experimentation, partial ureteral obstruction does not influence the level of kidney infection in terms of bacterial count, and the score of inflammation regardless of whether a virulent or low virulent strain was used. At long-term (42 days) experimentation, E. coli Mez was eliminated from all mice kidneys, but CFT073 persisted in almost all; obstruction did not influence the level of kidney infection with CFT073 but a significant difference of KWR and the inflammation and fibrosis score between obstructed and unobstructed kidneys was found (P = .0078; P = .036, respectively). Although the E. coli Mez strain did not persist in renal parenchyma, severe damage of the renal parenchyma was observed.
The proposed model is similar to the obstructive uropathy in children in which ureteral obstruction is present before the onset of infection. The association of obstruction and urinary infection impairs kidney growth and favors the occurrence of renal damage.
为了评估尿路感染和尿路梗阻对肾实质的影响，我们建立了一种伴有单侧部分梗阻性尿路病的小鼠上行性尿路感染模型。
6 周龄 CBA/J 小鼠接受右侧部分输尿管梗阻手术，并经尿道接受高毒力大肠杆菌 CFT073（高毒力株）或大肠杆菌 Mez（低毒力株）的挑战。研究了短期（72 小时）和长期（42 天）实验中尿液和肾脏的感染程度、肾脏炎症和纤维化评分以及肾脏重量比（KWR）。
在短期（72 小时）实验中，无论使用高毒力还是低毒力菌株，部分输尿管梗阻都不会影响肾脏感染的细菌计数和炎症评分。在长期（42 天）实验中，大肠杆菌 Mez 从所有小鼠肾脏中被清除，但 CFT073 几乎在所有小鼠肾脏中都存在；梗阻并不影响 CFT073 引起的肾脏感染程度，但在梗阻和非梗阻肾脏之间发现了 KWR 以及炎症和纤维化评分的显著差异（P =.0078；P =.036）。尽管大肠杆菌 Mez 株没有在肾实质中持续存在，但观察到肾实质严重损伤。
所提出的模型类似于儿童梗阻性尿路病，在感染发生之前就存在输尿管梗阻。梗阻和尿路感染的联合作用会损害肾脏生长，并有利于发生肾脏损伤。
