Ghanem Mona T M, Radwan Hany M A, Mahdy El-Sayed M, Elkholy Yehya M, Hassanein Heba D, Shahat Abdelaaty A
Department of Phytochemistry, National Research Centre, 12311 Dokki, Cairo, Egypt.
Pharmacognosy Res. 2012 Apr;4(2):104-8. doi: 10.4103/0974-8490.94735.
The study was designed to evaluate the antioxidant and hepatoprotective activities of the 80% methanolic extract as well as the ethyl acetate (EtOAc) and butanol (BuOH) fractions of the wild fennel (Foeniculum vulgare (Subsp; Piperitum)) and cultivated fennel (F. vulgare var. azoricum). In addition, quantification of the total phenolic content in the 80% methanol extract of fennel wild and cultivated herbs is measured.
An amount of 400 g of air dried powdered herb of wild and cultivated fennel were sonicated with aqueous methanol (80%), successively extracted with Hexane, EtOAc, and n-BuOH. The EtOAc and n-BuOH were subjected to repeated column chromatography on silica gel and Sephadex LH-20. The antioxidant effect was determined in vitro using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Hepatoprotective activity was carried out using a Wistar male rat (250-300 g). Total phenolic and flavonoid contents were determined as chlorogenic acid and rutin equivalents, respectively.
Two phenolic compounds, i.e., 3,4-dihydroxy-phenethylalchohol-6-O-caffeoyl-β-D-glucopyranoside and 3΄,8΄-binaringenin were isolated from the fennel wild herb, their structures were elucidated by spectral methods including 1D NMR, 2D NMR, and UV. The EtOAc and BuOH fractions of wild fennel were found to exhibit a radical scavenging activity higher than those of cultivated fennel. An in vitro method of rat hepatocytes monolayer culture was used for the investigation of hepatotoxic effects of the 80% methanol extract on the wild and cultivated fennel, which were >1000 and 1000 μg/mL, respectively. As well as, their hepatoprotective effect against the toxic effect of paracetamol (25 mM) was exerted at 12.5 μg/mL concentration.
Fennel (F. Vulgare) is a widespread plant species commonly used as a spice and flavoring. The results obtained in this study indicated that the fennel (F. vulgare) herb is a potential source of natural antioxidant. Two phenolic compounds, i.e. 3,4-dihydroxy-phenethylalchohol-6-O-caffeoyl-β-D-glucopyranoside (A) and 3΄,8΄-binaringenin (B) were isolated from the fennel wild herb for the first time.
本研究旨在评估野生茴香（Foeniculum vulgare (Subsp; Piperitum)）和栽培茴香（F. vulgare var. azoricum）80%甲醇提取物以及乙酸乙酯（EtOAc）和正丁醇（BuOH）萃取物的抗氧化和保肝活性。此外，还测定了野生和栽培茴香80%甲醇提取物中的总酚含量。
将400克野生和栽培茴香的风干粉末状草药用甲醇水溶液（80%）超声处理，依次用己烷、乙酸乙酯和正丁醇萃取。乙酸乙酯和正丁醇萃取物在硅胶和葡聚糖凝胶LH - 20上进行反复柱色谱分离。体外使用1,1 - 二苯基 - 2 - 苦基肼（DPPH）测定抗氧化效果。使用体重250 - 300克的雄性Wistar大鼠进行保肝活性实验。总酚和黄酮含量分别以绿原酸和芦丁当量来测定。
从野生茴香中分离出两种酚类化合物，即3,4 - 二羟基苯乙醇 - 6 - O - 咖啡酰基 - β - D - 吡喃葡萄糖苷和3΄,8΄ - 联芹菜素，通过包括一维核磁共振、二维核磁共振和紫外光谱等光谱方法阐明了它们的结构。发现野生茴香的乙酸乙酯和正丁醇萃取物表现出比栽培茴香更高的自由基清除活性。采用大鼠肝细胞单层培养的体外方法研究野生和栽培茴香80%甲醇提取物的肝毒性作用，其半数致死浓度分别>1000和1000 μg/mL。此外，它们在12.5 μg/mL浓度下对扑热息痛（25 mM）的毒性作用具有保肝效果。
茴香（F. Vulgare）是一种广泛分布的植物物种，常用作香料和调味品。本研究获得的结果表明，茴香（F. vulgare）草药是天然抗氧化剂的潜在来源。首次从野生茴香中分离出两种酚类化合物，即3,4 - 二羟基苯乙醇 - 6 - O - 咖啡酰基 - β - D - 吡喃葡萄糖苷（A）和3΄,8΄ - 联芹菜素（B）。
