Shahat Abdelaaty A, Alsaid Mansour S, Rafatullah Syed, Al-Sohaibani Mohammed O, Parvez Mohammad K, Al-Dosari Mohammed S, Exarchou Vassiliki, Pieters Luc
Pharmacognosy Department (Medicinal Aromatic & Poisonous Plants Research Centre), College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi Arabia.
Phytochemistry Department, National Research Centre, 33 El Bohouth St. (former El Tahrir st.) 12622, Dokki, Giza, Egypt.
BMC Complement Altern Med. 2016 Sep 8;16(1):351. doi: 10.1186/s12906-016-1318-3.
Consumption of plant-derived nutraceuticals and crude drugs in traditional medicine is widely believed to confer beneficial effects in thwarting the progression of cardiovascular diseases. Rhus tripartita (family Anacardiaceae) has been traditionally used to treat a wide range of ailments.
In the present study we investigated the protective effects of an alcoholic extract of the stem part of Rhus tripartita male genotype (RTSM) on experimentally induced myocardial injury in rats. To this end, cardiac injury was induced by administration of isoproterenol (ISO) and serum enzyme markers, lipid profiles and cardiac tissue redox status were determined following RTSM treatment (250 and 500 mg/kg).
As a result, RTSM treatment significantly mitigated ISO-triggered upregulation of cardiac-specific markers of injury creatine kinase and lactate dehydrogenase. RTSM treatment significantly attenuated ISO-induced increase in serum cholesterol and triglycerides as well alterations in serum lipoproteins. Determination of oxidative balance showed that RTSM treatment significantly blunted ISO-induced increase in malondialdehyde and decrease in nonprotein sulfhydryl in cardiac tissue. Six compounds were isolated and identified as gallocatechin 1, taxifolin 2, myricetin-3-O-β-glucoside 3, catechin 4, epicatechin 5, and 3',8-binaringenin 6. Compound 6 was isolated for the first time from the stem part of Rhus tripartita. Furthermore, RTSM treatment enhanced the survival fraction of cardiac cells exposed to oxidative stress in vitro.
We conclude that the antioxidant properties of RTSM treatment underpin its cardioprotective pharmacological effects, thus, providing biological evidence for the treatment of cardiovascular diseases using Rhus tripartita in indigenous medicine.
人们普遍认为，食用植物源营养保健品和传统医学中的草药对预防心血管疾病进展具有有益作用。盐肤木（漆树科）传统上用于治疗多种疾病。
在本研究中，我们研究了盐肤木雄性基因型茎部乙醇提取物（RTSM）对实验性诱导的大鼠心肌损伤的保护作用。为此，通过给予异丙肾上腺素（ISO）诱导心脏损伤，并在RTSM处理（250和500mg/kg）后测定血清酶标志物、血脂谱和心脏组织氧化还原状态。
结果表明，RTSM处理显著减轻了ISO引发的心脏特异性损伤标志物肌酸激酶和乳酸脱氢酶的上调。RTSM处理显著减轻了ISO诱导的血清胆固醇和甘油三酯升高以及血清脂蛋白的改变。氧化平衡测定表明，RTSM处理显著减弱了ISO诱导的心脏组织中丙二醛增加和非蛋白巯基减少。分离并鉴定出6种化合物，分别为表没食子儿茶素1、花旗松素2、杨梅素-3-O-β-葡萄糖苷3、儿茶素4、表儿茶素5和3',8-二氢杨梅素6。化合物6首次从盐肤木茎部分离得到。此外，RTSM处理提高了体外暴露于氧化应激的心脏细胞的存活分数。
我们得出结论，RTSM处理的抗氧化特性是其心脏保护药理作用的基础，从而为传统医学中使用盐肤木治疗心血管疾病提供了生物学证据。
