通过 Kutzneria spp. 744 中的 N5-羟基鸟氨酸生物合成哌嗪酸。
Biosynthesis of piperazic acid via N5-hydroxy-ornithine in Kutzneria spp. 744.
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA.
出版信息
Chembiochem. 2012 May 7;13(7):972-6. doi: 10.1002/cbic.201200054. Epub 2012 Apr 20.
Abstract
Which came first? We have investigated the biosynthesis of the piperazic acid (Piz) building blocks in the kutzneride family of metabolites. The flavin-dependent oxygenase KtzI was shown to convert ornithine to N(5)-OH-Orn. LC-MS/MS showed (13)C(5)-labeled versions of these two amino acids to be direct precursors of piperazic acid in vivo.
摘要
我们研究了库茨纳德里德代谢物家族中哌嗪酸（Piz）结构单元的生物合成途径。黄素依赖型加氧酶 KtzI 被证明能够将鸟氨酸转化为 N(5)-OH-Orn。LC-MS/MS 显示这两种氨基酸的 (13)C(5)-标记版本是体内哌嗪酸的直接前体。
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