State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, and Institute of Insect Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
Pest Manag Sci. 2012 Sep;68(9):1296-305. doi: 10.1002/ps.3297. Epub 2012 Apr 20.
Glutathione S-transferases (GSTs) have received considerable attention in insects for their roles in insecticide resistance. Laodelphax striatellus (Fallén) is a serious rice pest. L. striatellus outbreaks occur frequently throughout eastern Asia. A key problem in controlling this pest is its rapid adaptation to numerous insecticides. In this research, nine cDNAs encoding GSTs in L. striatellus were cloned and characterised.
The cloned GSTs of L. striatellus belonged to six cytosolic classes and a microsomal subgroup. Exposure to sublethal concentrations of each of the six insecticides, DDT, chlorpyrifos, fipronil, imidacloprid, buprofezin and beta-cypermethrin, quickly induced (6 h) up-expression of LsGSTe1. The expression of LsGSTs2 was increased by chlorpyrifos, fipronil and beta-cypermethrin. Furthermore, exposure of L. striatellus to fipronil, imidacloprid, buprofezin and beta-cypermethrin increased the expression of the LsGSTm gene after 24 or 48 h.
This work is the first identification of GST genes from different GST groups in Auchenorrhyncha species and their induction characteristics with insecticide types and time. The elevated expression of GST genes induced by insecticides might be related to the enhanced tolerance of this insect to insecticides and xenobiotics.
谷胱甘肽 S-转移酶（GSTs）在昆虫中因其在杀虫剂抗性中的作用而受到广泛关注。褐飞虱（Laodelphax striatellus）是一种严重的水稻害虫。褐飞虱在整个东亚地区频繁爆发。控制这种害虫的一个关键问题是其对众多杀虫剂的快速适应。在这项研究中，克隆并鉴定了褐飞虱中的 9 种 GSTs cDNA。
褐飞虱 GSTs 属于六个细胞溶质类和一个微粒体亚群。用亚致死浓度的六种杀虫剂（DDT、毒死蜱、氟虫腈、吡虫啉、噻嗪酮和β-氯氰菊酯）处理褐飞虱，可迅速诱导（6 小时） LsGSTe1 的高表达。氯氟氰菊酯、氟虫腈和β-氯氰菊酯增加了 LsGSTs2 的表达。此外，褐飞虱暴露于氟虫腈、吡虫啉、噻嗪酮和β-氯氰菊酯 24 或 48 小时后，LsGSTm 基因的表达增加。
这是首次在半翅目昆虫中鉴定出不同 GST 组的 GST 基因及其与杀虫剂类型和时间的诱导特征。杀虫剂诱导 GST 基因的高表达可能与该昆虫对杀虫剂和外来化合物的耐受性增强有关。
