Zhang Yueliang, Liu Baosheng, Zhang Zhichun, Wang Lihua, Guo Huifang, Li Zhong, He Peng, Liu Zewen, Fang Jichao
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing, China.
Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
J Econ Entomol. 2018 May 28;111(3):1382-1387. doi: 10.1093/jee/toy051.
Imidacloprid is a key insecticide used for controlling sucking insect pests, including the small brown planthopper (Laodelphax striatellus, Fallén) (Hemiptera: Delphacidae), an important agricultural pest of rice. A strain of L. striatellus (YN-ILR) developed 21-fold resistance when selected with imidacloprid on a susceptible YN strain. An in vitro study on piperonyl butoxide synergism indicated that enhanced detoxification mediated by cytochrome P450s contributed to imidacloprid resistance to some extent, and multiple P450 genes showed altered expression in the imidacloprid-resistant YN-ILR strain compared with the susceptible YN strain (CYP425B1-CYP6BD10 had 1.51- to 11.45-fold higher expression, CYP4CE2-CYP4DD1V2 had 0.12- to 0.57-fold lower expression). While there were no mutations in target nicotinic acetylcholine receptor (nAChR) genes, subunits of Lsα1, Lsβ1, and Lsβ3 in the YN-ILR strain showed 3.86-, 4.39-, and 2.59-fold higher expression and Lsa8 displayed 0.38-fold lower expression than the YN strain. Moreover, 21-fold moderate imidacloprid resistance in individuals of L. striatellus did not produce a fitness cost. The findings suggest that L. striatellus has the capacity to develop resistance to imidacloprid through P450 detoxification and potential target nAChR expression changes, and moderate imidacloprid resistance was not associated with a fitness cost.
吡虫啉是一种用于防治刺吸式害虫的关键杀虫剂，这些害虫包括灰飞虱（Laodelphax striatellus, Fallén）（半翅目：飞虱科），它是水稻的一种重要农业害虫。用吡虫啉对敏感的YN品系进行选育时，产生了一个灰飞虱品系（YN-ILR），其抗性提高了21倍。一项关于胡椒基丁醚增效作用的体外研究表明，细胞色素P450介导的解毒作用增强在一定程度上导致了对吡虫啉的抗性，与敏感的YN品系相比，多个P450基因在抗吡虫啉的YN-ILR品系中表达发生了变化（CYP425B1 - CYP6BD10的表达量高1.51至11.45倍，CYP4CE2 - CYP4DD1V2的表达量低0.12至0.57倍）。虽然靶标烟碱型乙酰胆碱受体（nAChR）基因没有突变，但YN-ILR品系中的Lsα1、Lsβ1和Lsβ3亚基的表达量比YN品系分别高3.86倍、4.39倍和2.59倍，而Lsa8的表达量比YN品系低0.38倍。此外，灰飞虱个体对吡虫啉产生的21倍中等抗性并未产生适合度代价。这些发现表明，灰飞虱有能力通过P450解毒作用和潜在的靶标nAChR表达变化来产生对吡虫啉的抗性，并且中等程度的吡虫啉抗性与适合度代价无关。
