Tao Caihong, Yang Shengrong, Zhang Junyan
School of Chemical and Biological Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
J Nanosci Nanotechnol. 2012 Jan;12(1):195-200. doi: 10.1166/jnn.2012.5749.
The fabrication of polymeric materials with ordered submicron-size void structures is potentially valuable for many applications such as catalysts, separation and adsorbent media. This paper reports the preparation of macroporous protein multilayer films with regular voids using silica nanospheres as templates. Both monodisperse silica colloids and highly ordered assembly silica multilayer films are used as templates to prepare microporous bovine serum albumin multilayer films with ruleless and ordered submicron-sized voids. Glutaraldehyde is used as a crosslinking agent to form a firm net-like protein film on the surface of silica templates. The microporous protein film is obtained after removing of silica templates. Compare with polymer film, protein film has good biocompatibility and biodegradability which will be beneficial to its biological applications.
制备具有有序亚微米级孔隙结构的聚合物材料在许多应用中具有潜在价值，如催化剂、分离和吸附介质等。本文报道了以二氧化硅纳米球为模板制备具有规则孔隙的大孔蛋白质多层膜。单分散二氧化硅胶体和高度有序组装的二氧化硅多层膜均被用作模板，以制备具有不规则和有序亚微米级孔隙的微孔牛血清白蛋白多层膜。戊二醛用作交联剂，在二氧化硅模板表面形成牢固的网状蛋白质膜。去除二氧化硅模板后得到微孔蛋白质膜。与聚合物膜相比，蛋白质膜具有良好的生物相容性和生物降解性，这将有利于其生物应用。
