Dental pulp stem cells from primary teeth quality analysis: laboratory procedures.

OBJECTIVES

To present details of isolation, processing and differentiation of stem cells from inflamed dental pulp of primary teeth.

MATERIALS AND METHODS

Tissue sample was collected from teeth indicated for a single visit pulp therapy. Samples were transported and processed in the laboratory which included culturing of cells, isolation and in vitro differentiation into multiple lineages. The results for the analysis of various cell surface markers used for dental pulp were compared with bone marrow.

RESULTS

There was no statistically significant difference found in the expression of various surface markers between dental pulp and bone marrow. The stem cells from dental pulp were differentiated into multiple lineages.

CONCLUSION

Isolation of cells from oral tissues is technique sensitive.