Merstorf Céline, Cressiot Benjamin, Pastoriza-Gallego Manuela, Oukhaled Abdel Ghani, Bacri Laurent, Gierak Jacques, Pelta Juan, Auvray Loïc, Mathé Jérôme
Laboratoire LAMBE (Equipe MPI) CNRS UMR 8587, Université d'Evry-val d'Essonne, Evry, France.
Methods Mol Biol. 2012;870:55-75. doi: 10.1007/978-1-61779-773-6_4.
We present here an overview on unfolding of biomolecular structures as DNA double strands or protein folds. After some theoretical considerations giving orders of magnitude about transport timescales through pores, forces involved in unzipping processes … we present our experiments on DNA unzipping or protein unfolding using a nanopore. We point out the difficulties that can be encountered during these experiments, such as the signal analysis problems, noise issues, or experimental limitations of such system.
我们在此概述生物分子结构的展开，如DNA双链或蛋白质折叠。在进行了一些理论考量，给出了通过孔的传输时间尺度、解链过程中涉及的力的量级之后……我们展示了使用纳米孔进行DNA解链或蛋白质展开的实验。我们指出了这些实验中可能遇到的困难，比如信号分析问题、噪声问题或此类系统的实验局限性。
