神经祖细胞中的鞘氨醇-1-磷酸信号传导

Sphingosine-1-phosphate signaling in neural progenitors.

作者信息

Callihan Phillip, Hooks Shelley B

机构信息

Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA, USA.

出版信息

Methods Mol Biol. 2012;874:193-200. doi: 10.1007/978-1-61779-800-9_15.

DOI:10.1007/978-1-61779-800-9_15

PMID:22528449

Abstract

Sphingosine-1-phosphate (S1P) and its receptors are important in nervous system development. Reliable in vitro human model systems are needed to further define specific roles for S1P signaling in neural development. We have recently reported that human embryonic stem cell-derived neuroepithelial progenitor cells (hES-NEP) express functional S1P receptors. These cells can be further differentiated to a neuronal cell type, and therefore represent a good model system to study the role of S1P signaling in human neural development. The following sections describe in detail the culture of hES-NEP cells and two assays to measure S1P signaling in these cells.

摘要

鞘氨醇-1-磷酸（S1P）及其受体在神经系统发育中起着重要作用。需要可靠的体外人类模型系统来进一步明确S1P信号在神经发育中的具体作用。我们最近报道，人类胚胎干细胞衍生的神经上皮祖细胞（hES-NEP）表达功能性S1P受体。这些细胞可以进一步分化为神经元细胞类型，因此代表了一个研究S1P信号在人类神经发育中作用的良好模型系统。以下部分详细描述了hES-NEP细胞的培养以及两种用于测量这些细胞中S1P信号的检测方法。