Shabankareh Hamed Karami, Seyedhashemi Seyed Behnam, Torki Mehran, Kelidari Hamidreza, Abdolmohammadi Alireza
Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah 74155, Iran.
Trop Anim Health Prod. 2012 Dec;44(8):1865-71. doi: 10.1007/s11250-012-0149-6. Epub 2012 Apr 17.
The objective of this study was to evaluate if treatment of equine chorionic gonadotrophin (eCG)-superovulated Sanjabi ewes with repeated administration of human chorionic gonadotropin (hCG) would increase the number of normal corpus luteum (CL) and serum progesterone concentrations and decrease the number of persistent follicles. The superovulated ewes were divided into four groups on day 0 (day of sponge removal); the ewes were treated by an intramuscular administration of 500 IU hCG on day 0 (Group I: n = 10), on days 0 and 1 (Group II: n = 10), or on days 0, 1, and 2 (Group III: n = 10) and no treatment for control group (n = 10). Blood samples were collected on days 0, 1, 2, 5, and 8 (day of slaughter), and serum progesterone concentrations were determined. According to progesterone concentrations, 50 (4/8) and 0 % of the ewes underwent premature luteal regression in the control group and the hCG groups, respectively. There were more CLs in Group III than in Group II and the control group. Ewes treated with hCG had a greater number of normal-looking CL. CL diameter was significantly greater in Group II and Group III than other groups. Total CL weight was significantly (P < 0.05) higher in Group III than in Group I and the control group. Number of persistent follicle and persistent follicle diameter were lower in control group compared to the other groups. Eight days after sponge removal, serum progesterone concentration was significantly higher in Group III than in Group I and the control group. The present results indicate that repeated administration of hCG supported CL formation, increased serum progesterone concentration, and prevented premature luteal regression in eCG-superovulated Sanjabi ewes.
本研究的目的是评估对经马绒毛膜促性腺激素（eCG）超排的桑贾比母羊反复注射人绒毛膜促性腺激素（hCG）是否会增加正常黄体（CL）的数量和血清孕酮浓度，并减少持久卵泡的数量。在第0天（取出海绵栓的当天）将超排母羊分为四组；第0天对母羊进行肌肉注射500 IU hCG（第一组：n = 10），第0天和第1天注射（第二组：n = 10），或第0天、第1天和第2天注射（第三组：n = 10），对照组不进行处理（n = 10）。在第0天、第1天、第2天、第5天和第8天（屠宰日）采集血样，并测定血清孕酮浓度。根据孕酮浓度，对照组和hCG组分别有50%（4/8）和0%的母羊发生过早黄体退化。第三组的CL数量多于第二组和对照组。用hCG处理的母羊有更多外观正常的CL。第二组和第三组的CL直径明显大于其他组。第三组的CL总重量显著高于第一组和对照组（P < 0.05）。对照组的持久卵泡数量和持久卵泡直径低于其他组。取出海绵栓8天后，第三组的血清孕酮浓度明显高于第一组和对照组。目前的结果表明，反复注射hCG可支持CL形成，增加血清孕酮浓度，并防止经eCG超排的桑贾比母羊过早黄体退化。
