School of Chemistry, Kings Building, West Mains Road, University of Edinburgh, Edinburgh, EH9 3JJ UK.
Macromol Rapid Commun. 2012 Jul 13;33(13):1114-8. doi: 10.1002/marc.201200193. Epub 2012 Apr 23.
Arrays of 84 polymer gradients, fabricated on a single glass microscope slide, were generated by inkjet printing, allowing a combination of high-throughput and true combinatorial methods. The gradual change of composition within the polymer gradients, consisting of two different monomers and a cross-linker, was validated by XPS and fluorescence analysis. Cellular screening of the gradients allowed the rapid identification of optimal polymer compositions for binding of the suspension cell line K562 and the adherent cell line HeLa. The polymers identified were identical to those identified by previous microarray data, providing proof of concept for the successful application of the polymer gradient arrays as a screening tool. In addition, the polymer gradients could be readily modified by conjugation enabling the generation of bio-molecule gradients.
通过喷墨打印技术在单个玻璃显微镜载玻片上制造了 84 个聚合物梯度阵列，实现了高通量和真正组合方法的结合。聚合物梯度内组成的逐渐变化，由两种不同的单体和交联剂组成，通过 XPS 和荧光分析进行了验证。对梯度的细胞筛选允许快速确定用于结合悬浮细胞系 K562 和贴壁细胞系 HeLa 的最佳聚合物组成。鉴定的聚合物与以前的微阵列数据鉴定的聚合物相同，为成功应用聚合物梯度阵列作为筛选工具提供了概念验证。此外，通过缀合可以很容易地修饰聚合物梯度，从而产生生物分子梯度。
