Kim Sung-Koo, Yang Hong, Pascual Juan M, De Vivo Darryl C
Department of Pediatrics, Hallym University College of Medicine, Seoul, Republic of Korea.
J Child Neurol. 2013 Jan;28(1):70-6. doi: 10.1177/0883073812440044. Epub 2012 Apr 24.
Glucose transporter 1 facilitates glucose transport across the blood-brain barrier. By increasing histone acetylation at the SLC2A1 promotor, valproic acid could increase SLC2A1 gene expression. This study was designed to evaluate the effects of valproic acid on glucose transport in astrocyte cultures derived from SLC2A1 heterozygous mice. Primary astrocyte cultures were prepared from the cerebral cortex of 1-day-old neonatal mice. Cultured astrocytes were incubated with valproic acid (0.05, 0.5, and 5 mM) for 48 hours. On day 3, the glucose uptake capacity of the astrocytes was measured by using (14)C-2-Deoxy-d-glucose under zero-trans conditions. The heterozygous astrocyte glucose uptake treated with valproic acid (0.05 and 0.5 mM) for 48 hours was significantly increased compared with the untreated control heterozygous astrocytes. Our findings demonstrate that valproic acid increased glucose transport capacity in SLC2A1 heterozygous cerebral astrocytes.
葡萄糖转运蛋白1促进葡萄糖穿过血脑屏障。通过增加SLC2A1启动子处的组蛋白乙酰化，丙戊酸可增加SLC2A1基因表达。本研究旨在评估丙戊酸对源自SLC2A1杂合小鼠的星形胶质细胞培养物中葡萄糖转运的影响。从1日龄新生小鼠的大脑皮质制备原代星形胶质细胞培养物。将培养的星形胶质细胞与丙戊酸（0.05、0.5和5 mM）孵育48小时。在第3天，在零转运条件下使用（14）C-2-脱氧-D-葡萄糖测量星形胶质细胞的葡萄糖摄取能力。与未处理的对照杂合星形胶质细胞相比，用丙戊酸（0.05和0.5 mM）处理48小时的杂合星形胶质细胞葡萄糖摄取显著增加。我们的研究结果表明，丙戊酸增加了SLC2A1杂合脑星形胶质细胞中的葡萄糖转运能力。
