National Engineering Laboratory for Industrial Enzymes, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Bioresour Technol. 2012 Jul;116:334-9. doi: 10.1016/j.biortech.2012.03.103. Epub 2012 Apr 5.
Highly efficient polymer-grade L-lactic acid production was achieved by an alkaliphilic strain Bacillus sp. WL-S20 using inexpensive peanut meal as nitrogen source and sodium hydroxide as neutralizing agent. In multi-pulse fed-batch fermentation of Bacillus sp. WL-S20, a L-lactic acid concentration of 225 g/l with a yield of 99.3% was obtained. In single-pulse fed-batch fermentation, a concentration of 180 g/l was obtained with a yield of 98.6%. No D-isomers of lactic acid were detected. The production of a high concentration of optically pure L-lactic acid by alkaliphilic Bacillus sp. WL-S20, combined with a low-cost nutrient and environment-friendly NaOH-based process, represent a potentially novel way for L-lactic acid production at an industrial scale.
嗜碱性芽孢杆菌 WL-S20 利用廉价的花生粕作为氮源，氢氧化钠作为中和剂，高效生产高分子量 L-乳酸。在芽孢杆菌 WL-S20 的多脉冲分批发酵中，获得了 225 g/L 的 L-乳酸浓度，产率为 99.3%。在单脉冲分批发酵中，获得了 180 g/L 的浓度，产率为 98.6%。未检测到乳酸的 D-异构体。嗜碱性芽孢杆菌 WL-S20 生产高浓度光学纯 L-乳酸，结合低成本营养物和环保的基于 NaOH 的工艺，为工业规模生产 L-乳酸提供了一种潜在的新方法。
