Bączek Tomasz, Olędzka Ilona, Konieczna Lucyna, Kowalski Piotr, Plenis Alina
Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland.
ScientificWorldJournal. 2012;2012:268120. doi: 10.1100/2012/268120. Epub 2012 Mar 12.
The validated micellar electrokinetic chromatography (MEKC) was proposed for the determination of five steroid hormones in human urine samples. That technique allowed for the separation and quantification of cortisol, cortisone, corticosterone, testosterone, and epitestosterone and was sensitive enough to detect low concentrations of these searched steroids in urine samples at the range of 2-300 ng/mL. The proposed MEKC technique with solid-phase extraction (SPE) procedure was simple, rapid, and has been successfully applied as a routine procedure to analyze steroids in human urine samples. The MEKC method offered a potential in clinical routine practice because of the short analysis time (8 min), low costs, and simultaneous analysis of five endogenous hormones. Due to its simplicity, speed, accuracy, and high recovery, the proposed method could offer a tool to determine steroid hormones as potential biomarkers in biomedical investigations, what was additionally revealed with healthy volunteers.
验证后的胶束电动色谱法（MEKC）被用于测定人尿液样本中的五种甾体激素。该技术能够分离并定量皮质醇、可的松、皮质酮、睾酮和表睾酮，并且灵敏度足以检测尿液样本中浓度低至2 - 300 ng/mL的这些目标甾体激素。所提出的采用固相萃取（SPE）程序的MEKC技术简单、快速，已成功作为常规程序应用于分析人类尿液样本中的甾体激素。MEKC方法由于分析时间短（8分钟）、成本低且能同时分析五种内源性激素，在临床常规实践中具有潜力。由于其简单、快速、准确且回收率高，该方法可为生物医学研究中作为潜在生物标志物的甾体激素测定提供一种工具，健康志愿者的情况进一步证明了这一点。
