Millucci Lia, Ghezzi Lorenzo, Bernardini Giulia, Braconi Daniela, Tanganelli Piero, Santucci Annalisa
Dipartimento di Biotecnologie, Università degli Studi di Siena, Via Fiorentina 1, 53100 Siena, Italy.
ScientificWorldJournal. 2012;2012:293863. doi: 10.1100/2012/293863. Epub 2012 Mar 12.
Atrial natriuretic peptide (ANP), whose amyloid is responsible of isolated atrial amyloidosis (IAA), is known to play an important role in the pathophysiology of congestive heart failure (CHF). We provide here the microscopic examination of atrial biopsies from 36 young (mean 40 years) CHF patients distinguished in idiopathic dilated cardiomyopathy (DC) affected and hypertrophic Cardiomyopathy (HC) affected, endorsing the presumptive association of early CHF with IAA. We utilized a multiple method, using Congo red (CR) staining, CR fluorescence (CRF), and immunohistochemistry to assess the presence of IAA in CHF. Immunostaining showed a moderate deposition of IAA in the atrium surrounding working myocardium with small intracellular deposits. Our findings suggest a monitoring of young CHF cases for the development of IAA. Our study also demonstrated how the concurrent use of immunohistochemistry, CR, and CRF may greatly enhance the detection of low-grade amyloid deposits.
心房利钠肽（ANP）的淀粉样蛋白是孤立性心房淀粉样变性（IAA）的病因，已知其在充血性心力衰竭（CHF）的病理生理学中起重要作用。我们在此提供了对36名年轻（平均40岁）CHF患者心房活检的显微镜检查，这些患者分为特发性扩张型心肌病（DC）患者和肥厚型心肌病（HC）患者，支持早期CHF与IAA之间的推测关联。我们采用多种方法，使用刚果红（CR）染色、CR荧光（CRF）和免疫组织化学来评估CHF中IAA的存在。免疫染色显示IAA在工作心肌周围的心房中有中度沉积，并伴有小的细胞内沉积物。我们的研究结果表明，应对年轻CHF病例进行监测，以观察IAA的发展情况。我们的研究还证明，免疫组织化学、CR和CRF的联合使用可大大提高对低度淀粉样沉积物的检测。
